不同碘剂对缺碘大鼠视网膜抗氧化能力的影响  

Effects of supplementation of different kinds of iodine on the antioxidative ability of retina in iodine deficient rats

作　　者：吴粤秀[1] 李丽娟[1] 陈贯一[2] 赵文德[2] 邱明才[1] 

机构地区：[1]天津医科大学总医院内分泌科,300052 [2]天津市内分泌研究所

出　　处：《中华眼科杂志》2003年第8期495-498,共4页Chinese Journal of Ophthalmology

摘　　要：目的　研究不同剂量碘剂对碘缺乏大鼠机体的作用 ,探讨补碘剂量和剂型 2个因素对碘缺乏大鼠视网膜抗氧化能力和脂质过氧化的影响。方法　将 12 8只碘缺乏的Wistar大鼠随机分为4个组 :适碘碘酸钾组、适碘碘化钾组、高碘碘酸钾组及高碘碘化钾组 ,分别补充不同剂量和不同剂型的碘 ,补碘 2 2周后检测血清总三碘甲腺原氨酸、总四碘甲腺原氨酸水平 ,测定视网膜组织中谷胱甘肽过氧化物酶含量、超氧化物歧化酶活力、总抗氧化能力及脂质过氧化物丙二醛含量。结果　剂量因素对血清总三碘甲腺原氨酸影响显著 ,但对血清总四碘甲腺原氨酸无显著影响 ;剂型因素对甲状腺激素水平无影响。剂量和剂型 2个因素对视网膜组织抗氧化能力均有显著影响。剂量因素对超氧化物歧化酶活力有显著影响 ,2个适碘组超氧化物歧化酶活力大于 2个高碘组 ;剂型对视网膜总抗氧化能力有显著影响 ,2个碘化钾组总抗氧化能力大于 2个碘酸钾组。各组谷胱甘肽过氧化物酶含量比较 ,差异无显著意义 (P >0 0 5 ) ,但趋势与前两者一致 ,即 2个适碘组大于 2个高碘组 ,2个碘化钾组大于 2个碘酸钾组。各组脂质过氧化物丙二醛含量无剂量与剂型差异。结论　碘剂的剂量和剂型 2个因素影响碘缺乏大鼠视网膜的抗氧化能力 ;适碘组视网膜抗氧化能力大于高碘组 ;?Objective To study the effects of iodine supplementation (in different kinds and doses) on the antioxidative ability of retina in iodine deficient rats. Methods One hundred and twenty eight iodine deficient Wistar rats were randomly divided into four groups, normal dose of iodate, normal dose of iodide, high dose of iodate and high dose of iodide. Concentration of serum thyroid hormones, including total TT 3 and total TT 4, were estimated by radioimmunoassay. GSH-Px、SOD、TAOC activities and MDA content in the retina were determined using biochemical methods in the 22nd week of iodine supplementation. Results Statistical analysis showed that a significant difference in TT 3 level of serum was observed between animals treated with different doses. Serum TT 3 level in the groups treated with high doses was significantly higher than those with normal doses. However, no statistical difference could be detected at TT 4 level between animals treated with different doses. Different kind of iodine did not affect the level of thyroid hormones. Statistical analysis showed that a difference in SOD activity of retina was observed between animals treated with different doses. SOD activity in the groups with normal doses was significantly higher than that in groups with larger doses. Retina TAOC activity was significantly higher in groups treated with iodide than that in groups of iodate. Although there was no statistical difference in GSH-Px activity between different groups, it showed the same tendency as the SOD and TAOC activities, i.e. GSH-Px activity in the groups of normal doses was higher than that in the groups of high doses. GSH-Px activity in groups of iodide was higher than that in the groups of iodate. There was no significant difference in MDA content among these four groups. Conclusions Different iodine and doses have certain effects on the antioxidative ability of retina in iodine deficient rat. The rats supplemented potassium iodide at normal dose showed higher antioxidative ability of the retina than

关 键 词：碘剂 大鼠 视网膜 抗氧化能力 脂质过氧化作用 

分 类 号：R965[医药卫生—药理学]