5种肽激素在黄鳝消化道内分泌细胞中的分布特点  

THE DISTRIBUTON CHARACTERISTIC OF FIVE PEPTIDE HORMONES IN THE ENDOCRINE CELLS IN DIGESTIVE TRACT OF MONOPTERUS ALBUS

作　　者：方之平[1] 潘黔生[2] 赵雅心[1] 潘玉善[1] 王翔凌[1] 

机构地区：[1]华中农业大学动物兽医学院,武汉430070 [2]华中农业大学水产学院,武汉430070

出　　处：《解剖学报》2003年第5期560-562,共3页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (30 0 70 5 90 )

摘　　要：目的　探索黄鳝消化道黏膜中 5种内分泌细胞的定位及分布。　方法　采用免疫细胞化学技术中的链霉亲合素 生物素过氧化物酶复合物 (SABC)法。　结果　在黄鳝消化道黏膜中至少分布着 5种免疫活性内分泌细胞 ,它们是 :胃泌素 (Gastrin ,Gas)、生长抑素 (Somatostatin ,Som)、五羟色胺 (5 Hydroxytryptamine ,5 HT)、胰岛素(Insulin ,Ins)和神经丝蛋白 免疫活性内分泌细胞 (Neurofilament immunoreactivecell,NF)。其中Gas和Som细胞分布在食道复层扁平上皮与杯状细胞之间 :贲门处有少量Gas细胞分布 ,胃底上皮和胃腺中有大量的Som细胞分布 ,而Ins、NF和 5 HT细胞则分别分布在幽门上皮和幽门腺管上。黄鳝的肠中则未见到上述任何一种免疫活性阳性反应。另外 ,胰高血糖素在黄鳝消化道任何部位均未见到免疫活性阳性反应。以上各免疫活性内分泌细胞均为深褐色 ,形状不规则 ,胞突较短而粗 ,胞核呈空泡状 ,常夹于食道和胃上皮之间或分布于腺上皮之间 ,将胞突伸向胃腔或腺腔。　结论　作为最低等脊椎动物 ,黄鳝的消化道具有复杂的内分泌功能。Objective To investigate the localization and distribution of the five endocrine cells in the digestive tract mucosa of ricefield eel(Monopterus albus). Methods Using immunocytochemical technique of strept avidin-biotin-peroxidase complex(SABC) were used. Results At least 5 kinds of immunoreactive endocrine cells distributed in the digestive tract mucosa of M.albus. They were gastrin(Gas),somatostatin(Som),5-hydroxytryptamine(5-HT),insulin(Ins),and neurofilament (NF) immunoreactive endocrine(IRE) cells.Gas and Som-IRE cells distributed between stratified squamous epithelium and goblet cell in esophagus. A large number of Gas-IRE cells were found between gastric fundus epithelium and gastric glands, and only a few in the carcia. Ins, 5-HT and NF-IRE cells distributed in the epithelium pylorus and pyloric glandular tube respectively. No any immunoreactive positive reaction was found in the gut of M.albus.In addition, immunoreactive positive reaction of glucagons was not found in whole digestive tract.All immunoreactive endocrine cells were dark brown in color.Their morphology was irregular, cytoplasmic process was shorter and thicker, their nucleus showed an empty bubble.They distributed between esophageal epithelium and gastric epithelium or glandular epithelium, and cytoplasmic process extended to the gastric lumen and glandular cavity.Conclusion There is a complex endocrine function of the digestive system in ricefield eel (M.albus) at the lowest vertebrate.

关 键 词：肽激素 黄鳝 消化道 内分泌细胞 分布特点 

分 类 号：S961.1[农业科学—水产养殖]