Simultaneous Determination of Tetramethylpyrazine and Aspirin in a New Compound Formulation by Liquid Chromatography  

液相色谱法同时测定复方制剂中川芎嗪和阿司匹林的含量(英文)

作　　者：王鹏[1] 齐美玲[2] 周莉[3] 方林[1] 

机构地区：[1]沈阳药科大学制药工程学院,沈阳110016 [2]北京理工大学理学院化学系,北京100081 [3]沈阳华泰药物研究所,沈阳110016

出　　处：《Journal of Chinese Pharmaceutical Sciences》2004年第1期58-62,共5页中国药学（英文版）

摘　　要：Aim To establish a reversed-phase liquid chromatographic (LC) method forsimultaneous determination of tetramethylpyrazine (TMP) and aspirin in a new compound formulation.Methods Chromatographic separation of the two drugs was achieved on a Diamonsil C_(18) column, usinga binary mixture of methanol-1.5% acetic acid (35:65, V/V, pH = 3.1) as mobile phase at a flow rateof 1.0 mL·min^(-1). Results Separation was completed in less than 12 min. Benzoic acid was used asthe internal standard. Recoveries at levels corresponding to 80 % to 120 % of the label claim ofthe formulation ranged from 99.6 to 100.3 % for aspirin and from 99.9 to 101.3% for TMP. The linearrange was 12.6 - 150.9 μg·mL^(-1)(r= 0.9997, n = 5) for aspirin and 25.0- 300.0 μg·mL^(-1) (r =0.9999, n = 5) for TMP. Conclusion The method developed can be used for the simultaneousdetermination of TMP and aspirin in pharmaceutical preparations.目的　建立一种反相液相色谱法用于新复方制剂中川芎嗪和阿司匹林含量的同时测定。方法　采用DiamonsilC18色谱柱 ,以甲醇 -1 5%醋酸 (3 5∶65,V V ,pH =3 1)为流动相 ,流速为 1 0mL·min- 1。结果　色谱测定可在 12min内完成。苯甲酸为内标。在标示量 80 -12 0 %的范围内 ,川芎嗪和阿司匹林的回收率范围分别为 99 6-10 0 3 %和 99 9-10 1 3 %。川芎嗪和阿司匹林的线性范围分别为 2 5 0 -3 0 0 0 μg·mL- 1(r=0 9999,n =5)和 12 5-150 9μg·mL- 1(r=0 9997,n =5)。

关 键 词：liquid chromatography TETRAMETHYLPYRAZINE ASPIRIN ASSAY 

分 类 号：R927.2[医药卫生—药学] O657.72[理学—分析化学]