增强型场效应管NH_4^+敏尿素传感器的实验研究  

Experimental research of NH_4^+ sensitive urea sensor with reinforcement ISFET

作　　者：蒲晓允[1] 李招权[1] 黄辉[1] 邓均[1] 潘静[1] 张正元[2] 李俊[2] 

机构地区：[1]第三军医大学医学检验系临床检验学教研室,重庆400038 [2]电子工业部第24研究所,重庆400070

出　　处：《重庆医学》2004年第4期565-567,共3页Chongqing medicine

摘　　要：目的　结合平面半导体技术及生物化学原理 ,制作NH+ 4 敏感的尿素传感器 ,并对其检测性能进行评价。方法　采用平面半导体技术制作ISFET ,在栅上固化脲酶得到NH+ 4 敏尿素传感器 ,并在ISFET自身稳定性、传感器响应时间、检测范围及保存时间几个方面对该传感器进行评价。结果　ISFET自身性能较稳定 ,传感器响应时间 <6 0s,尿素检测范围为 1 0～ 1 0 0 0mg/L ,保存7d内该传感器检测性能较稳定。结论　NH+ 4Objective To fabricate the NH + 4 sensitive urea sensor with reinforcement ISFET by conjugating the plane semiconductor technique with biochemical theory and to evaluation it′s detecting behavior.Method We got ISFET using the plane semiconductor technique and then urease was solidified on the gate of ISFET.Next,the stability of ISFET itself,the response time,the detecting range and the effect of preservation time on the sensor were tested respectively.Result The stability of ISFET was fine.As for the sensor,it′s response time was less than sixty seconds,the urea detecting range was 10-1 000mg/L and it had stable detecting behavior within 7 days.Conclusion The urea sensor in our experiments has some practical applied values.

关 键 词：ISFET NH4^+ 尿素 传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]