钙结合蛋白在老年人海马CA1和CA3区的分布  

The distribution of calbindin D 28K in senile hippocampal CA1 and CA3

作　　者：胡亚卓[1] 张红红[1] 许丹[1] 朱明伟[1] 王鲁宁[1] 

机构地区：[1]解放军总医院老年医学研究所,北京100853

出　　处：《中华老年心脑血管病杂志》2004年第2期101-103,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家重点基础研究发展规划基金 (G2 0 0 0 0 5 70 0 5 )

摘　　要：目的　探讨老年人海马中钙结合蛋白 (CalbindinD 2 8K ,CaBP)的分布及表达 ,并分析其在人衰老过程中对神经元的生理、病理变化的影响。方法　收集 12例 6 0岁以上老年人非脑部疾病尸检左侧大脑半球的海马组织 ,经病理常规处理后 ,切片 ,用免疫组织化学ABC染色法检测海马CA1与CA3区CaBP的分布。结果　CaBP阳性产物出现在神经元的细胞质中 ,CA1区的CaBP免疫阳性细胞数多于CA3区。结论　CaBP在老年人海马CA1、CA3中均有表达 ,但有差别。提示CaBP在老年人海马的功能活动中可能起重要作用。Objective To investigate the distribution of calbindin D 28K (CaBP) in senile hippocampus and to analyse the effect of CaBP on change of physiology and pathology of neurons during aging.Method The hippocampus samples of left cerebral hemisphere were processed with pathological routine and sliced after autopsy in 12 cases over 60 years of age without brain disorders.Distribution of CaBP in hippocampus CA1 and CA3 areas was observed with immunohistochemical ABC staining.Results CaBP immunoprecipitates were shown to appear in neuronal cytoplasm.CaBP-immunoreactive cells were more numerous in CA1 of hippocampus than in CA3.Conclusion These data suggest that CaBP might play an important role in function of hippocampus.

关 键 词：钙结合蛋白质类 海马 免疫组织化学 

分 类 号：R361[医药卫生—病理学]