小鼠肝癌H-22细胞膜相关抗原肽提取物的抑瘤作用及其免疫机制的研究  

Inhibitory Effect of Tumor-associated Antigen Peptides Prepared from H-22 Hepatocarcinoma on Tumor Growth in Mice and its Immune Mechanisms

作　　者：孙祖玥[1] 吕喆[1] 刘淑春[1] 龚守良[1] 李修义[1] 赵勇[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021

出　　处：《肿瘤防治杂志》2003年第6期573-576,共4页China Journal of Cancer Prevention and Treatment

基　　金：吉林省卫生厅资助课题

摘　　要：目的 :应用小鼠肝癌H 2 2细胞膜相关抗原肽 (TAP)提取物免疫小鼠 ,观察其对小鼠自身移植肿瘤生长及免疫学参数的影响 ,为制备肿瘤疫苗提供实验依据。方法 :采用微酸洗脱法制备相对分子质量≤ 3× 10 3的细胞膜TAP提取物 ,皮下免疫小鼠 ,检测胸腺细胞周期时相和T细胞亚组百分数的变化 ;并检测脾细胞ConA反应性、IL 2和IFN γ分泌活性、CTL杀伤活性的变化 ,以及脾脏T细胞CD3、CD6 9和TCR的表达和抑瘤效应。结果 :TAP提取物免疫后 ,移植肿瘤的发生率降低 ,平均出现时间延迟 ,生长速度减慢 ;同时 ,脾细胞CD6 9表达增加 ,ConA反应性、IFN γ和IL 2分泌活性和CTL杀伤活性不同程度增强 ;胸腺细胞CD3表达显著增高、CD+ 4和CD+ 8T细胞百分数也不同程度增高。结论 :小鼠肝癌H 2 2细胞膜TAP提取物具有免疫原性 ,能有效激发免疫系统功能 。Objective To investigate the effect of tumor associated peptide (TAP) extract on the tumor development in mice autotransplanted with H 22 hepatocarcinoma and its immune functions Methods Mild acid elution method was applied to prepare TAP extract (MW≤3×10 3) from the tumor cell membrane After immunization with TAP extract,thymocyte percentages of the cell cycle phase and the percentages of T lymphocyte subsets were determined with flow cytometry (FCM).The reactivity of splenocytes to Con A,the secreting activities of interleukin 2 (IL 2) and interferon (IFN γ) of splenocytes and the cytotoxicity of CTL were tested The expression of CD 3,CD 69 and TCRαβ of T lymphocytes were detected with FCM Its anti tumor effect of TAP extract on tumor development was observed Results After immunization with TAP extract,the incidence of the transplanted tumor reduced,the average tumor appearing time delayed and the growth speed of the tumor decreased in mice The expression of CD 69 in the splenocytes and the reactivity of them to Con A in TAP immune group increased significantly as compared with that in control.At the same time,IL 2 and IFN γ secreting activities of splenocytes increased,and the toxic effects of CTL in spleen enhanced significantly.In addition,the expression of CD 3 in thymus and spleen enhanced significantly,and the percentages of CD + 4 and CD + 8 T lymphocytes in thymus increased at different levels in thymus Conclusions The TAP extract has immunogenicity and could efficiently activate immune system and inhibit tumor development The inhibitory effects of TAP on tumor could be achieved through the enhancements of immune functions

关 键 词：肝肿瘤 免疫学 抗原 肿瘤 肽类 

分 类 号：R735.7[医药卫生—肿瘤]