骨碎补总黄酮对去卵巢大鼠骨密度和细胞因子IL-6、IL-4、TNFα水平的影响  

Effect of Osteopractic Total Flavone on Density of Bones of Rats without Ovaria and Levels

作　　者：谢雁鸣[1] 许勇钢[1] 赵晋宁[1] 王智[1] 李谨[2] 

机构地区：[1]中国中医研究院西苑医院,北京100091 [2]北京积水潭医院,北京100035

出　　处：《中国中医基础医学杂志》2004年第1期34-37,共4页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的 :通过去卵巢大鼠骨质疏松模型 ,研究骨碎补总黄酮对骨密度和血清IL 6、IL 4、TNFα水平的影响。方法 :采用X线骨密度测量仪测定大鼠股骨、腰椎骨密度 ;应用放免法 ,通过自动酶免分析仪测定血清IL 6、IL 4、TNFα水平 ,在 5 5 0nm光下读取OD值 ,应用标准曲线计算含量。结果 :骨碎补总黄酮能显著提高大鼠股骨、腰椎骨密度 ,作用明显优于骨松宝对照组。骨碎补总黄酮能调节细胞因子IL 6、IL 4、TNFα的水平 ,其中中剂量组IL 6、TNFα较模型组明显降低 ,骨碎补总黄酮三个剂量组和阳性对照组IL 4含量明显高于模型组。结论 :骨碎补总黄酮能明显提高去卵巢大鼠的骨密度 ,对血清TNFα、IL 6水平有抑制作用 ,对IL 4分泌有促进作用。Objective:To study the effect of the osteopractic total flavone on the density of bones of rats without ovaria and the levels of IL-6,IL-4 and TNFα a serums through the osteoporosis models of the rats.Methods:Determine the density of the rats' femora and lumbar vertebra with the Bones' Density X-ray Measuring Instrument;determine the levels of IL-6,IL-4 and TNFα a serums with the Automatic Ferments Immunity Analyzing Instrument by means of radio-immunity,read the OD figures of these serums under the 550nm light,and calculate their content.Results:The osteopractic total flavone can remarkably increase the density of rats' fermora and lumbar vertebra.Its effect is much superior to that of the GUSONGBAO contrast group.It also can regulate the levels of IL-6,IL-4 and TNFα a cellular factors.Compared with the model group,the content of IL-6 and TNFα a in the medium-dose group decreaseds remarkably(P<0.05).The content of IL-4 in the three osteopractic total flavone groups and positive contrast group is much higher than in the model group(P<0.01).Conclusion:The osteoparactic total flavone can notably increase the density of rats without ovaria,control the levels of IL-6,IL-4 and TNFα a serums,and accelerate the secretion of IL-4 werum.

关 键 词：骨碎补总黄酮 去卵巢大鼠 骨密度 细胞因子 IL-6 IL-4 TNFΑ 骨质疏松 

分 类 号：R681[医药卫生—骨科学] R-332[医药卫生—外科学]