用SCGE法观察砷、氟、黄绿青霉素和T-2毒素引起的SGC-7901细胞基因组DNA损伤  

The study of DNA damage induced by arsenic, fluoride,CIT and T-2 toxin in cultured SGC-7901 cells by SCGE

作　　者：姜树林[1] 青雪梅[1] 孙殿军[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心,黑龙江哈尔滨150086

出　　处：《中国地方病学杂志》2003年第4期289-291,共3页Chinese Jouranl of Endemiology

基　　金：黑龙江省杰出青年科学基金资助项目 ( J0 1 - 0 4 )

摘　　要：目的　观察砷、氟、黄绿青霉素和 T-2毒素对 SGC-790 1细胞基因组 DNA的损伤作用及特点。方法　染毒剂量 10μmol/ L ,染毒后 4h应用单细胞凝胶电泳技术结合“彗星图像分析系统”检测 DNA损伤。结果　Na As O2 组尾长明显大于对照组 ;Na F组拖尾率显著高于对照组 ;CIT组总拖尾率、尾长和尾动量均高于对照组 ;而 T-2毒素组各指标均高于对照组。结论　 Na As O2 、Na F、CIT和 T-2毒素均可引起 DNA损伤 ,但各有特点 ,说明它们引起Objective To study the features of DNA damage induced by arsenic, fluoride, citreoviridin (CIT) and T-2 toxin in cultured SG C-7901 cells.Methods Single cell gel electrophoresis(SCGE) and Komet Image Analysis System (KIAS) were used to measure the DNA damage of the cells t reated for 4 hours with 10 μmol/L sodium arsenite, sodium fluoride, CIT or T- 2 toxin,respectively.Results The tail lengths of damaged DNA were significan tly greater in the sodium arsenite group than those in the control group; the ta iled cell percentage in the sodium fluoride group was significantly higher than that in the control group; CIT significantly increased the tailed cell percentag e, tail length and tail moment; whereas in the T-2 toxin group, the tailed cell percentage, tail DNA, tail length and tail moment were all increased. Conclusions Sodium arsenite, sodium fluoride, CIT and T -2 toxin are all DNA damage inducers, but the DNA damages induced by them show different features, so their mechanisms of inducing DNA damage may be different.

关 键 词：SCGE法 砷 氟 黄绿青霉素 T-2毒素 SGC-79O1细胞基因 DNA损伤 公共卫生学 

分 类 号：R363[医药卫生—病理学]