Sep15人硒蛋白基因的克隆  

Cloning of a Human Selenoprotein Gene

作　　者：南克俊[1] 李春丽[1] 魏永长[1] 隋晨光[1] 景昭[1] 秦海霞[1] 赵利军 

机构地区：[1]西安交通大学第一医院肿瘤内科,陕西西安710061 [2]Georgia大学,美国athensga30602

出　　处：《肿瘤防治杂志》2003年第10期1030-1032,共3页China Journal of Cancer Prevention and Treatment

摘　　要：目的 :克隆人 15× 10 3硒蛋白 (15× 10 3selenoprotein ,Sep15 )基因。方法 :体外培养H9T淋巴细胞 ,裂解细胞获取总RNA ,RT PCR扩增人Sep15基因 ,再将其克隆到T载体上 ,序列测定并酶切鉴定重组体。结果 :RT PCR扩增得到大小约 12 4 4bp左右的基因片段 ,序列分析结果与Genbank上提交的人Sep15基因序列完全一致。NotI酶切鉴定得到 30 15bp左右的T载体片段及 12 4 4bp左右的人Sep15基因片段 ,与预期值相符。结论Objective To colon the 15×10 3 selenoprotein gene and construct the T vector of this gene.Methods H9 human T cells were cultured in vitro in routine condition.The mRNA was isolated from the cells.The cDNA library of 15×10 3 selenoprotein was constructed by RT PCR.The gene was coloned into T vector,then it was sequenced and the recombinant vector was identified by enzymatic digestion.Results Special strap about 1 244 bp of 15×10 3 selenoprotein gene was obtained.Sequence analysis showed its sequence was absolutely consistent with the sequence of 15×10 3 selenoprotein gene that had published on Genbank.After the enzymatic digestion with Not I,two straps about 1 244 bp and 3 015 bp of 15×10 3 selenoprotein gene and T vector were obtained.It was consistent with the anticipated value.Conclusion Human 15×10 3 selenoprotein gene was obtained successfully.

关 键 词：蛋白质类 分析 克隆 硒 

分 类 号：R73-3[医药卫生—肿瘤]