胃癌相关抗原表位模拟短肽的筛选  

Screening of bioactive peptide that mimic the epitope of gastric cancer associated antigen

作　　者：韩全利[1] 丁杰[1] 巩爱春[2] 喻召才[1] 乔泰东[1] 陈宝军[1] 张学庸[1] 樊代明[1] 

机构地区：[1]第四军医大学西京医院消化疾病研究所,陕西西安710032 [2]解放军第473医院妇产科,甘肃兰州730070

出　　处：《细胞与分子免疫学杂志》2003年第3期308-310,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No .39870 742 )

摘　　要：目的 :筛选能模拟胃癌相关抗原表位的功能短肽。方法 :利用离子交换层析 ,对抗胃癌相关抗原单克隆抗体 (mAb)进行纯化 ,并以此mAb为靶标 ,将其包被于ELISA板上 ,以噬菌体随机 12肽库对其进行 3轮亲和筛选。结果 :从噬菌体随机12肽库中 ,筛选到 12个噬菌体阳性克隆。结论 :获得了具有模拟胃癌相关抗原表位的阳性噬菌体克隆 。AIM: To screen bioactive peptides that mimic the epitope of gastric cancer associated antigen. METHODS: Anti gastric cancer monoclonal antibody (mAb) MG7 was purified by ion chromatography, and then coated on ELISA plate. By using the mAb as selective molecular, a 12 meres phage displaying peptide library was biopanned, and the positive clones were selected.RESULTS: 12 positive phage clones were selected. CONCLUSION: A candidate mimic epitope of gastric cancer associated antigen was identified, which provided foundation for developing tumor peptide vaccine.

关 键 词：表位 噬菌体表面呈现 随机短肽库 

分 类 号：R730.3[医药卫生—肿瘤] R735.2[医药卫生—临床医学]