大鼠硒性白内障晶状体的超微结构  

Ultrastructure of the selenite-induced cataract lenses in rats

作　　者：严宏[1] 惠延年[1] 黄晓峰[2] 黄梅[2] 

机构地区：[1]第四军医大学西京医院眼科,陕西西安710033 [2]第四军医大学基础部电子显微镜中心,陕西西安710033

出　　处：《第四军医大学学报》2003年第21期1947-1949,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :观察大鼠硒性白内障晶状体的超微结构 .方法 :出生 8～ 1 0d的SD大鼠连续 5次皮下注射亚硒酸钠生理盐水 (每日 2mmol·L-1 ) ,2只正常和 2只白内障的晶状体按透射电镜标本制备成超薄切片 ,在电镜下观察细胞的超微结构 .结果 :硒性白内障大鼠的晶状体在电镜下细胞间连接破坏 ,胞质密度降低 ,胞质轻度液化 ,线粒体囊状变性 ,胞质内出现大量数目不等、分布不均匀的高密度球形结构 .结论AIM: To study the ultrastructural changes of the selenite induced cataract lenses in rat. METHODS: Selenite cataract was induced in 8-10 days old Sprague Dawley rats by 5 subcutaneous injections of sodium selenite (2 mmol·L -1 daily). Ultrathin sections of 2 normal and 2 cataractous lenses were prepared to investigate the ultrastructural changes by transmission electron microscopy. RESULTS: Ultrastructural observation of selenium cataractous lenses revealed destruction of intercellular junction, low cytoplasmic density and slight liquefaction, cystic degeneration of mitochondria, and high density round substances with different amount and uneven distribution in the cytoplasm. CONCLUSION: The high density round substances may contribute to formation of selenite induced cataract.

关 键 词：硒性白内障 晶状体 透射电子显微术 大鼠 超微结构 高密度球形结构 

分 类 号：R776.1[医药卫生—眼科]