短暂性前脑缺血后大鼠海马各区NMDA受体亚单位NR2A和NR2B mRNA的表达变化  

ALTERATIONS OF NMDA RECEPTOR SUBUNITS NR2A AND NR2B mRNA EXPRESSION IN HIPPOCAMPUS OF RAT FOLLOWING TRANSIENT FOREBRAIN ISCHEMIA

作　　者：刘志安[1] 徐铁军[1] 张凤真[1] 王梅申[1] 彭裕文[2] 

机构地区：[1]徐州医学院解剖学和神经生物学教研室,徐州221002 [2]复旦大学上海医学院解剖学与组织胚胎学系,上海200032

出　　处：《神经解剖学杂志》2004年第2期153-157,共5页Chinese Journal of Neuroanatomy

基　　金：江苏省自然科学基金 (BK99192 )资助项目

摘　　要：应用原位杂交组织化学和图像处理与分析的方法 ,观察短暂性前脑缺血 (15 m in)再灌注 (0 .5 h～ 7d)大鼠海马各区NR2 A和 NR2 B m RNA的表达变化 ,探讨二者在缺血性脑损伤中的作用。结果发现 ,缺血后 ,NR2 A和 NR2 B m RNA在海马各区呈现出一种相对一致的表达。在海马 CA1 区 ,NR2 A和 NR2 B m RNA的表达分别在缺血再灌 6h和 12 h降至低谷 (P<0 .0 5 ) ,然后表达开始回升 ,在缺血再灌 48h都升至高峰 (P<0 .0 5 ) ,之后表达再次下降 ,直至缺血后 7d(P<0 .0 5 ) ;在海马 CA3区 ,二者的表达变化规律与 CA1 区相似 ,不同的是表达变化的幅度明显减小。在齿状回 ,缺血后 0 .5 h～ 72 h,NR2 A和 NR2 B m RNA的表达未见显著性变化 ,72 h后表达开始下降 ,直至缺血后 7d(P<0 .0 5 )。以上结果提示 ,短暂性前脑缺血后 ,NR2 A和 NR2 B m R-NA在大鼠海马各区表达变化的模式不同 。By in situ hybridization (ISH) and image processing and analysis system, we observed the alterations of NR2A and NR2B mRNA expression in hippocampus of rat following transient forebrain ischemia. The ischemia(15 min) reperfusion(0.5 h-7 d) (IR) animal model was created by the four artery occlusion (4AO). The results showed that the expression of NR2A and NR2B mRNA had relative concordance in regions of hippocampus after IR. In the CA 1, the expression of NR2A and NR2B mRNA dropped to the lowest at 6 h and 12 h of IR(P<0.05). Then the expression started to recover and both rised to the highest at 48 h of IR(P<0.05). And then the expression started to decrease again until the 7 d of IR(P<0.05). In the CA 3, the pattern of NR2A and NR2B mRNA expression was similar to that in the CA 1. The variousness was that the range of the expression change obviously decreased. And in the dentate gyrus, before 72 h of IR, the expression of NR2A and NR2B mRNA had no significant change and then the expression also started to decrease until the 7 d of IR(P<0.05). The results indicate that the alterations of NR2A and NR2B mRNA expression showed a different pattern in regions of rat hippocampus after IR. This difference hint that the alterations of NR2A and NR2B mRNA expression after IR may contribute to selective vulnerable phenomenon and delayed cell death of hippocampus.

关 键 词：脑缺血 大鼠 海马 NMDA受体亚单位 NR2A NR2B mRNA 表达 

分 类 号：R361[医药卫生—病理学]