消化法培养成年猴许旺细胞  

Cell culture of monkey's Schwann cell by digestion

作　　者：易建华[1] 刘小林[1] 朱家恺[1] 向剑平[1] 王建云[1] 

机构地区：[1]中山大学第一附属医院黄埔院区骨科,广州市510080

出　　处：《中华显微外科杂志》2004年第1期40-42,F004,共4页Chinese Journal of Microsurgery

基　　金：国家自然科学基金资助项目 (30 1 70 962 );广东省自然科学基金资助项目 (990 0 67)

摘　　要：目的　探讨猴许旺细胞在标准条件下的培养、扩增与传代。　方法　根据Wallerian变性后许旺细胞增殖的原理 ,利用双酶消化法及阿糖胞苷 (Ara c)抑制成纤维细胞生长分裂的方法获取许旺细胞。用S 10 0抗体鉴定所培养的细胞并用MTT法检测这些细胞的增殖能力。　结果　猴许旺细胞培养 15d后可达 5× 10 6,纯度为 92 % ,MTT测定证实该细胞生长良好。　结论　神经预变性后 ,通过消化法和Ara c处理能获取量大纯度高、活力良好的许旺细胞 ,可用于组织工程化人工神经的种子细胞。Objective To evaluate the culture and subculture method Schwann cells of monkey in a standard condition. Methods According to the principle that Schwann cells could proliferate fater Wallerian degeneration,monkey Schwann cells were cultured through double-enzyme digestion and fibroblasts' suppression with Ara-c.Schwann cells were identified by immunohistochemistry with S-100 antibody and their proliferating function was assessed by MTT assay. Results There were 5×106 monkey Schwann cells in 15 days with a purity of 92% and positive staining by S-100,and it was proved by MTT assay that the proliferation of Schwann cells was healthy and normal. Conclusions Abundant,highly pure and active Schwann cells were obtained by double-enzyme digestion and Ara-c procession after predegeneration of nerve.These cells could be used as seed cells in tissue engineering bioartificial nerve.

关 键 词：消化法 许旺细胞 细胞培养 阿糖胞苷 MTT法 组织工程 人工神经 

分 类 号：R-331[医药卫生]