香烟烟雾提取物对人胚肺成纤维细胞增殖及转化生长因子β1、表皮生长因子表达的影响  

Effects of cigarette smoke extract on the proliferation and the expression of TGF-β_1 and EGF in human embryonic lung fibroblasts

作　　者：葛晓娜[1] 熊密[1] 黄召[2] 

机构地区：[1]华中科技大学同济医学院病理学系,武汉430030 [2]华中科技大学同济医学院附属协和医院

出　　处：《中华结核和呼吸杂志》2004年第1期51-54,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　观察香烟烟雾提取物 (CSE)对人胚肺成纤维细胞 (HELF)增殖及转化生长因子 β1(TGF β1)、表皮生长因子 (EGF)表达的影响。方法　用含不同浓度CSE[分别为 1∶50 (CSE1组 )、1∶2 5(CSE2 组 )和 1∶10 (CSE3 组 )倍稀释 ]的培养基作用于体外培养的HELF ,对照组 (C组 )不含CSE。观察不同浓度的CSE对细胞增殖及增殖细胞核抗原 (PCNA)水平的影响。用逆转录 聚合酶链反应 (RT PCR)方法检测各组细胞TGF β1mRNA表达量。用免疫组织化学方法检测各组细胞TGF β1和EGF蛋白的表达量。结果　经CSE作用后 ,随着CSE浓度的增高 ,HELF增殖呈逐渐减弱的趋势 ,PCNA表达阳性率在CSE1组为 2 6 94%、CSE2 组为 2 0 0 6%、CSE3 组为 11 0 7% ,均比C组 (3 6 0 5% )明显下降(P <0 0 5)。RT PCR和免疫组织化学染色结果发现 :低浓度的CSE (1∶50和 1∶2 5)使HELF的TGF β1mRNA及蛋白表达明显增强 ,与C组比较差异有显著性意义 (P <0 0 5)。免疫组织化学染色显示 :较高浓度的CSE(1∶2 5和 1∶10 )可引起HELF的EGF蛋白表达减弱 ,与C组比较差异有显著性 (P <0 0 5)。结论　CSE可抑制HELF的增殖 。Objective To observe the effects of cigarette smoke extract(CSE) on the proliferation and the expression of TGF-β 1 and EGF in human embryonic lung fibroblasts (HELF) Methods The cultures of HELF were incubated with CSE(1∶50, 1∶25 and 1∶10) for examining the effects of CSE on the proliferation and the proliferating cell nuclear antigen(PCNA) levels in HELF The total RNA was extracted from the cells incubated with CSE at different doses The expression levels of TGF-β 1 mRNA were evaluated by reverse transcription-polymerase chain reaction(RT-PCR) The mRNA levels of TGF-β 1 were corrected by GAPDH transcripts TGF-β 1 and EGF protein levels were determined by immunocytochemistry Results The proliferation of HELF was inhibited by CSE in a dose-dependent manner CSE also decreased the PCNA levels of HELF at all doses( P< 0 05) CSE stimulated TGF-β 1 mRNA and protein expression of HELF at lower concentrations (1∶50 and 1∶25, P< 0 05) TGF-β 1 mRNA and protein production were not increased by CSE at a higher concentration(1∶10, P> 0 05) Decreased expression of EGF protein was observed in HELF treated by CSE(1∶25 and 1∶10, P< 0 05) Conclusions CSE can inhibit the proliferation of HELF and the effect may involve the abnormal expression of TGF-β 1 and EGF in HELF

关 键 词：香烟烟雾提取物 人胚肺 成纤维细胞增殖 转化生长因子Β1 表皮生长因子 表达 吸烟 肺气肿 

分 类 号：R363[医药卫生—病理学]