含CpG基序的寡核苷酸介导的脐血抗白血病细胞作用的研究  

Study of antileukemic effect of CpG-oligodeoxynucleotides treated cord blood

作　　者：陈春燕[1] 贾继辉[2] 潘祥林[1] 周亚滨[2] 赵蔚明[2] 

机构地区：[1]山东大学第二医院血液肿瘤科,济南250033 [2]山东大学医学院微生物学教研室,250012

出　　处：《中华血液学杂志》2004年第1期13-16,共4页Chinese Journal of Hematology

摘　　要：目的　观察脐血淋巴细胞经人工合成的含不同CpG基序的寡核苷酸 (CpG ODN)作用后抗白血病细胞的效应。方法　设计合成了四种含不同CpG基序的寡核苷酸 (CpG ODN1 、CpG ODN2 、CpG ODN3和CpG ODN4 )、不含CpG基序的寡核苷酸 (nonCpG ODN)和含甲基化CpG基序的寡核苷酸(ZpG ODN) ,将其分别作用于脐血淋巴细胞 ,用MTT法测定经寡核苷酸作用后的脐血淋巴细胞对白血病K5 6 2细胞的杀伤作用 ,同时用流式细胞术分析了对脐血淋巴细胞刺激作用最强的CpG ODN3作用于脐血淋巴细胞后表面分化抗原的变化。结果　不同的CpG基序对脐血淋巴细胞有不同程度的免疫刺激作用 ,其中CpG ODN3作用后脐血NK细胞对K5 6 2细胞杀伤率最强 ,且在一定范围内呈剂量依赖性变化 ,经CpG ODN3作用后脐血淋巴细胞表面分化抗原CD3、CD4 、CD1 9、CD56 表达率分别为 (6 0 .6±7 9) %、(40 .2± 3.5 ) %、(2 2 .4± 1.9) %、(15 .5± 3.1) % ,均较作用前升高 (P值均 <0 .0 5 )。结论　不同的CpG基序对脐血淋巴细胞免疫刺激作用存在差异 ,优选后的CpG ODN3可较大程度地激活脐血淋巴细胞抗K5 6 2细胞效应 。Objective To observe the antileukemic effect of lymphocytes from cord blood treated by CpG-oligodeoxynucletides(CpG-ODN). Methods Lymphocytes from cord blood were exposed to different oligodeoxynucleotides containing a panel of CpG-ODN and were cultured with K562 cells. The cytotoxic effects were detected by MTT method. Immunological markers of cord blood treated by CpG-ODN_3 which showed highest activity were measured with flow cytometry. Results Different CpG-motifs have different immunostimulatory activity and CpG-ODN_3 has the highest one. After treated by CpG-ODN_3,NK killing activity to K562 cells increased in a dose-dependent manner,and CD_3,CD_4,CD_ 19 and CD_ 56 increased to (60.6±7.9)%,(40.2±3.5)%,(22.4±1.9)% and (15.5±3.1)%,respectively. Conclusion CpG-ODN could reinforces the immunological competence of cord blood lymphocytes and their effects on K562 cells. This provides a new approach to reinforce the antitumor effects of cord blood.

关 键 词：CPG基序 寡核苷酸 脐血 白血病细胞 抗肿瘤效应 细胞免疫功能 

分 类 号：R733.7[医药卫生—肿瘤]