Therapeutic effects and molecular mechanisms of anti-fibrosis herbs and selenium on rats with hepatic fibrosis  

作　　者：Yu-TongHe Dian-WuLiu Li-YuDing QingLi Yong-HongXiao 

机构地区：[1]DepartmentofEpidemiology,HebeiMedicalUniversity,Shijiazhuang050017,HebeiProvince,China

出　　处：《World Journal of Gastroenterology》2004年第5期703-706,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by the Natural Science Foundation of Hebei Province,No.302489

摘　　要：AIM:To study the therapeutic effects of anti-fibrosis herbs and selenium on hepatic fibrosis induced by carbon tetrachloride (CCl4) in rats and the underlining molecular mechanisms.METHODS:Fifty-three Wistar rats were randomly divided into:normal control group, model control group, colchicine group, anti-fibrosis herbs group (AF group) and anti-fibrosis herbs plus selenium group (AS group).The last four groups were administered with CCl4 at the beginning of experiment to induce hepatic fibrosis. Then colchicine, anti-fibrosis herbs and selenium were used to treat them. The normal control group and the model control group were given normal saline at the same time. At the end of the 6^th week, rats in each group were sacrificed. Blood and tissue specimens were taken. Serum indicators (ALT, AST, HA, LN) were determined and histopathological changes were graded. Lymphocyte CD4 and CD8 were examined by flow cytometry. Expression of TGF-β1 and NF-κB was detected by immunohistochemistry and expression of TGF-β1 mRNA was detected by semiquantified RT-PCR.RESULTS: Histological grading showed much a smaller degree of hepatic fibrogenesis in AS group and AF group than that in colchicine group and model control group.The serum content of ALT, AST, HA and LN in AF group and AS group were significantly lower than that in colchicine group (ALT:65.8±26.5, 67.3±18.4 and 96.2±20.9 in AF, AS and colchicine groups respectively; AST: 150.8±34.0, 154.6±27.3 and 215.8±24.6 respectively; HA: 228±83, 216±58 and 416±135 respectively; LN:85.9±15.0, 80.6±18.6 and 106.3±14.2 respectively) (P<0.05). The level of CD4 and CD4/CD8 ratio in AF group and AS group was significantly higher that those in cochicine group (CD4:50.8±3.8, 52.6±3.4 and 40.2±2.1 in AF, AS and colchicine groups respectively;CD4/CD8 ratio:1.45,1.46 and 1.26, respectively (P<0.05).The expression level of NF-κB and TGF-β1 in the liver tissues of AF and AS treatment groups was markedly decreased compared with that in cochicine group, and TGF-β1 mRNA was alsAIM:To study the therapeutic effects of anti-fibrosis herbs and selenium on hepatic fibrosis induced by carbon tetrachloride (CCl_4) in rats and the underlining molecular mechanisms. METHODS:Fifty-three Wistar rats were randomly divided into:normal control group,model control group,colchicine group,anti-fibrosis herbs group (AF group) and anti-fibrosis herbs plus selenium group (AS group).The last four groups were administered with CCl_4 at the beginning of experiment to induce hepatic fibrosis.Then colchicine,anti-fibrosis herbs and selenium were used to treat them.The normal control group and the model control group were given normal saline at the same time.At the end of the 6^(th) week,rats in each group were sacrificed.Blood and tissue specimens were taken.Serum indicators (ALT,AST,HA,LN) were determined and histopathological changes were graded.Lymphocyte CD_4 and CD_8 were examined by flow cytometry.Expression of TGF-β_1 and NF-κB was detected by immunohistochemistry and expression of TGF-β_1 mRNA was detected by semi- quantified RT-PCR. RESULTS:Histological grading showed much a smaller degree of hepatic fibrogenesis in AS group and AF group than that in colchicine group and model control group.The serum content of ALT,AST,HA and LN in AF group and AS group were significantly lower than that in colchicine group (ALT:65.8±26.5,67.3±18.4 and 96.2±20.9 in AF,AS and colchicine groups respectively;AST:150.8±34.0,154.6±27.3 and 215.8±24.6 respectively;HA:228±83,216±58 and 416±135 respectively;LN:85.9±15.0,80.6±18.6 and 106.3±14.2 respectively) (P<0.05).The level of CD_4 and CD_4/CD_8 ratio in AF group and AS group was significantly higher that those in cochicine group (CD_4:50.8±3.8,52.6±3.4 and 40.2±2.1 in AF,AS and colchicine groups respectively; CD_4/CD_8 ratio:1.45,1.46 and 1.26,respectively (P<0.05). The expression level of NF-κB and TGF-β_1 in the liver tissues of AF and AS treatment groups was markedly decreased compared with that in cochicine group,and TGF-β_1 mRNA was also markedly

关 键 词：肝纤维化 四氯化碳 动物模型 细胞因子 药物疗法 分子机制 

分 类 号：R575.2[医药卫生—消化系统]