激活的脐血树突状细胞对肿瘤细胞的直接杀伤作用  

The Direct Cytotoxic Effect of Activated Dendritic Cell Derived from Cord Blood on Tumor Cells

作　　者：石军[1] 池田和真[2] 藤井伸治[1] 李晓[1] 浦权[1] 

机构地区：[1]上海交通大学附属第六医院血液科,上海200233 [2]日本冈山大学附属医院第二内科

出　　处：《现代免疫学》2004年第2期89-92,共4页Current Immunology

基　　金：上海市青年科技启明星课题资助项目 [沪科 ( 2 0 0 2 ) 2 70号 ];上海市卫生局青年基金资助项目 ( 0 2 4Y14 )

摘　　要：探索脐血树突状细胞 (DC)在γ干扰素 (IFN γ )及细菌脂多糖 (LPS )激活前后对肿瘤细胞杀伤活性的差异。分离健康脐血单核细胞 ,用重组粒单细胞集落刺激因子 (GM CSF )、白介素 4 (IL 4 )和α肿瘤坏死因子α (TNF α )诱导为DC。于诱导第11天在合成培养基中加入LPS或IFN γ继续培养 12h ,将其激活。用流式细胞仪检测DC表面共刺激分子的改变 ,以明确LPS或IFN γ对DC的不同刺激作用 ;同时 ,以恶性血液病细胞株Jurkat及Daudi为靶细胞 ,以不同效靶比 (E∶T )与DC共同培养 18h ,采用51Cr释放试验检测DC激活前后抗肿瘤活性的差异。结果 (1)LPS及IFN γ可不同程度地上调DC表面CD86、CD80、CD83及CD1a的表达 ,尤以LPS刺激组明显 ;(2 )DC在未加刺激因子前能有效杀伤Jurkat细胞 ,在效靶比为 2 0∶1时 ,LPS或IFN γ可使其杀伤活性进一步提高至 5 0 0 %、 36 9% ,均与未加刺激因子前有显著差异 (P <0 0 0 1,P <0 0 2 5 ) ;(3)在效靶比为 2 0∶1时 ,LPS可使DC对Daudi的杀伤率提高至 19 8% (P <0 0 2 5 ) ,而未加刺激因子组及IFN γ刺激组DC对Daudi在任何效靶比均未显示明显的杀伤作用。LPS或IFNTo investigate the differences of tumocidal activities of dendritic cells (DC) derived from cord blood before and after the stimulation with IFN-γ or LPS. The mononuclear cells collected from cord blood of healthy individuals were cultured with IL-4,GM-CSF and TNF-α for 11 days to induce DC.After another 12 hours of culture,in the presence or abscnce of IFN-γ or LPS, the changes of costimulating molecules,such as CD86,CD80,CD83 and CD1a were analyzed with flow cytometry. Jurkat cells and Daudi cells used as target cells were cocultured with DC at various effector target ratios for 18 hours,and then the percentage of tumorcidal activities were measured by means of 51Cr release assay. It was found that LPS and IFN-γ could up-regulate the expression of costimulating molecules at certain degree. The significant changes was detected after stimulation with LPS. Although these DC showed tumorcidal activities on Jurkat cells before stimulation,moreover,both LPS and IFN-γ enhanced the cytotoxicity of DC to 50% and 38.9% respectively at a effector-target ration of 20∶1,and all superior to that of the unstimulated DC. In addition,LPS could induce DC to enhance the cytotoxicity on Daudi cells up to 19.8%, while the unstimulated or IFN-γ stimulated DC did not show any cytotoxic effect on Daudi cell at any effector-target radio. It concludes that DC derived from cord blood activated by LPS or IFN-γ exhibit specific tumorcidal activities against Jurkat and Daudi cells.

关 键 词：脐血 树突状细胞 肿瘤细胞 细胞杀伤作用 Γ干扰素 细菌脂多糖 

分 类 号：R730.3[医药卫生—肿瘤]