中国猪种DQB新等位基因的克隆和分析  

Molecular Cloning and Characterization of New SLA DQB Alleles and Their Signifi-cance

作　　者：陈福祥[1] 谢晋[1] 张勇[1] 周光炎[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025

出　　处：《现代免疫学》2004年第2期93-98,共6页Current Immunology

基　　金：国家自然科学基金资助重大项目 (批准号 :3 9993 43 0 2 )

摘　　要：克隆和序列分析中国猪种DQB基因cDNA ,为猪异种器官移植的免疫识别机制的研究提供基础。采用RT PCR方法扩增猪DQB基因cDNA ,克隆入测序载体 ,然后进行测序及其序列分析。结果获得具有阅读框架的三个结构和已有序列不同的DQB新等位基因 ,基因序列号分别为AY10 2 4 76、AY10 2 4 77和AY10 2 4 78,其长度为 786个核苷酸 ,除末端终止密码外 ,编码 2 6 1个氨基酸残基。发现了三个猪DQB新等位基因 。To study the mechanism of xenotransplantal rejection, class II DQB genes of swine leukocyte antigens (SLA) in three Chinese pig strains GXP, GZP and YNP were cloned, sequenced and analysed. RT-PCR was performed to proliferate SLA-DQB genes, which were cloned into pGEM-T Easy vector for sequencing and analysis. It was found that the three allelic sequences we examined were not identical to those reported, with their accession numbers from GenBank as AY102476, AY102477 and AY102478 respectively. Thus three new alleles of SLA-DQB genes were obtained. Analysis of the amino acid sequences of swine, human and mouse DQB (or equivalent) also indicated that GXPDQB, GZPDQB and YNPDQB were more similar to human DQB than mouse H-2-Aβ, which might provide an understanding of how the immune system evolved.

关 键 词：等位基因 克隆 DQB基因 异种器官移植 免疫识别机制 RT-PCR 排斥反应 

分 类 号：R392.4[医药卫生—免疫学]