小鼠肝癌细胞移植瘤内淋巴管的形态与亚细胞结构  

THE MORPHOLOGICAL OBSERVATION OF LYMPHATICS IN TRANSPLANT TUMOR IN MICE CAUSED BY H22

作　　者：李晓冬[1] 赵玲辉[1] 张雅芳[1] 高伟东[2] 李玉兰[1] 刘慧冬[1] 

机构地区：[1]哈尔滨医科大学人体解剖学教研室,哈尔滨150086 [2]黑龙江省电力医院,哈尔滨150086

出　　处：《解剖学报》2004年第2期194-197,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金 ( 3 0 2 70 697) ;黑龙江省自然科学基金 (D0 0 2 6)

摘　　要：目的　观察小鼠肝癌细胞所致小鼠移植瘤内淋巴管形态学改变 ,探讨移植瘤内是否有淋巴管新生。方法　将小鼠肝癌 (H2 2 )腹水型瘤株细胞接种于昆明小鼠腋部皮下 ,2周时摘取肿瘤 ,采用HE染色观察癌肿的发展 ,光镜下用 5′ 核苷酸酶 碱性磷酸酶双重染色法观察毛细淋巴管 ;半薄切片筛选毛细淋巴管 ;应用透射电镜观察毛细淋巴管内皮细胞超微结构。　结果　动物模型显示 2周时小鼠腋部可见明显肿块。 5′ 核苷酸酶 碱性磷酸酶双重染色法可见中心区无毛细淋巴管 ,周边部可见稀疏的染成棕黄色的毛细淋巴管 ,染成蓝色的血管较为多见。透射电镜下 ,周边区可见毛细淋巴管 ,亚细胞结构发生损伤。　结论　小鼠肝癌细胞所致小鼠移植瘤内存在新生毛细淋巴管。Objective To study the morphological changes of lymphatics in transplant tumor of mice. Methods H22 cells were inoculated under the armpit skin of KM mice. Tumor was obtained two weeks after inoculation. HE staining was used to observe the development of tumor. 5'-nase-alpase double staining was performed to observe blood vessels and lymphatics. Ultrastructures of lymphatics were observed with electron-microscopy. Results Tumor occurred in all the mice. Under microscope there was none of lymphatics in the center of the tumor, a few lymphatics and a lot of blood vessels could be seen in periphery of tumor. Under TEM damage of the organelles could be seen in the endothelial cell of lymphatics. Conclusion Neonatal lymphatics can be seen in transplant tumor caused by H22.

关 键 词：小鼠 肝癌细胞 移植瘤 淋巴管 形态 亚细胞结构 

分 类 号：R730.2[医药卫生—肿瘤]