胃癌前病变p21^(ras),c-erbB-2,p53表达与中医证候的关系  

Relationship between expression of p21^(ras), c-erbB-2 and p53 and TCM syndrome in gastric precancerous lesions

作　　者：胡玲[1] 劳绍贤[1] 

机构地区：[1]广州中医药大学脾胃研究所,广东省广州市510405

出　　处：《世界华人消化杂志》2004年第2期262-265,共4页World Chinese Journal of Digestology

基　　金：广州中医药大学科学研究与发展总体规划重点及广东省中医药管理局资助课题;No.GH0016 300011~~

摘　　要：目的:观察胃癌癌前病变p21ras,c-erbB-2,p53癌基因蛋白表达与中医证候的关系.方法:经胃镜及病理证实为胃癌癌前病变的病例共40例,其中中度异型增生24例,重度异型增生9例,不完全性结肠化生7例;中医辨证属脾胃气阴两虚兼气滞者10例,兼胃热者12例,兼血瘀者18例.所有胃黏膜活检标本采用抗生蛋白链菌素-生物素免疫组织化学标记的方法作p21ras、c-erbB-2、p53表达的检测.结果:p21ras,c-erbB-2,p53在胃癌癌前病变中有过表达,且其表达随着病变的进展而升高,但中、重度异型增生及不完全性结肠上皮化生胃黏膜之间的表达差异无显著性(P>0.05).在不同的兼证中,p21ras,p53的表达兼血瘀者大于兼胃热、气滞者(P<0.01),c-erbB-2的表达兼血瘀、胃热者大于兼气滞者(P<0.05).结论:p21ras,c-erbB-2,p53癌基因蛋白在胃癌癌前病变中有过表达,其表达与不同中医兼证有关,可能有一定的证候特异性.AIM: To explore the relationship between p21ras, c-erbB-2, p53 protein expression and TCM syndrome in gastric precancerous lesions (GPL). METHODS: Forty cases with endosmotically and pathologically confirmed GPL were studied, including 24 cases of moderate dysplasia of gastric mucosa, 9 cases of severe dysplasia, 7 cases of incomplete colonic intestinal metaplasia. By the differential diagnosis of TCM, pi-wei deficiency of Qiand Yin associated stagnation of Qi, stomach-heat, and blood stasis were 10,12 and 18 cases respectively. Expression of p21ras, c-erbB-2 and p53 proteins was detected by the LSAB immunohistochemical method. RESULTS: Overexpression of p21ras, c-erbB-2 and p53 proteins was found in GPL, and gradually increased with the progress of lesions; but among gastric mucosa of the mederate, severe dysphasia and incomplete colonic intestinal metaplasia, there are no differences in the expression of the genes (P >0.05). Among the differential associated symptoms and signs, the expression of p21ras and p53 oncogene proteins was the blood stasis > stomach-heat and Qi stagnation (P <0.01), and the expression of c-erbB-2 oncogene protein was the blood stasis and stomach-heat >Qi stagnation(P<0.05). CONCLUSION: Overexpression of p21ras, c-erbB-2 and p53 proteins is found in GPL. The expression is related with the differentially associated symptoms and signs.

关 键 词：基因表达 中医证候 胃黏膜 胃癌癌前病变 GPL 肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]