当归多糖对大鼠乙酸性结肠炎的保护作用  

Protective effects of Angelica sinensis polysaccharides on acetic acid-induced rat colitis

作　　者：刘少平[1] 董卫国[1] 余保平[1] 罗和生[1] 于皆平[1] 吴东方[2] 

机构地区：[1]武汉大学人民医院消化内科,湖北省武汉市430060 [2]武汉大学人民医院药学部,湖北省武汉市430060

出　　处：《世界华人消化杂志》2004年第2期367-370,共4页World Chinese Journal of Digestology

基　　金：湖北省科技攻关项目 No.2001AA308B~~

摘　　要：目的:研究当归多糖对大鼠乙酸性结肠炎损伤的保护作用及其初步机制.方法:建立大鼠乙酸性结肠炎模型.实验设正常对照组,模型对照组;阳性药物对照组(5氨基水杨酸100mg/kg);当归多糖给药组(250,500,1000mg/kg),每天灌肠给药一次,用药7d.评价大鼠结肠黏膜损伤指数(CMDI)及粪便隐血实验(OBT),检测结肠组织髓过氧化物酶(MPO),SOD活性,MDA、NO含量和TGF-β、EGF表达水平,并作病理组织学观察.结果:ASP灌肠明显降低模型组大鼠结肠显著升高的CMDI、OBT评分,MPO活性、MDA、NO含量(CMDI:2.1±0.8,1.8±0.6,1.4±0.7vs2.9±0.6;OBT:3.1±1.3,2.7±1.1,2.2±1.2vs3.8±0.8;MPO:77.2±23.6,72.5±16.8,61.3±19.2vs98.1±26.9;MDA:44.26±10.25,38.72±14.84,31.59±12.68vs31.59±12.68;NO:0.252±0.041,0.223±0.037,0.217±0.032vs0.331±0.092,P<0.05或P<0.01),明显升高模型组大鼠结肠显著降低的SOD活性与TGF-β表达水平(SOD:30.16±2.88,31.27±2.73,33.52±2.81vs28.33±1.17:TGF-β:0.136±0.031,0.153±0.036,0.169±O.029vs0.105±0.021,P<0.05或P<0.01),亦显著上调EGF:鞑(EGF:0.178±0.021,0.195±0.031,0.191±0.022vs0.151±0.026,P<0.05或P<0.01).ASP用药呈一定量效关系.SF灌肠亦改善模型组大鼠结肠病理组织学表现.结论:ASP灌肠明显缓解乙酸性结肠炎大鼠结肠损伤,机制与促生长因子、抗氧化和一定的抗炎作用相关.AIM: To study the protective effects of Angelica sinensis polysaccharides (ASP) on colon injury in acetic acid-induced rat colitis and its mechanism. METHODS: The colitis model of rats was produced by intracolon enema with acetic acid. The experimental animals were divided into 6 groups: normal, model, 5-ASA (100 mg/kg), and ASP (250, 500,1 000 mg/kg), and treated intracolonically with saline, 5-ASA, and ASP respectively once a day for 7 days. The colon mucosa damage index (CMDI) and occult blood test (OBT) were evaluated. The activities of MPO and SOD, the contents of MDA and NO, the expression levels of TGF-β and EGF in the colon tissue were detected. H-E stained section was also observed. RESULTS: Intracolon enema with ASP decreased the significanctly elevated extents of CMDI, OBT and levels of MPO, MDA, and NO in the model group (CMDI: 2.1±0.8, 1.8±0.6,1.4±0.7 vs 2.9±0.6; OBT: 3.1±1.3,2.7±1.1, 2.2±1.2 vs 3.8±0.8; MPO: 77.2±23.6, 72.5±16.8, 61.3±19.2 vs 98.1±26.9; MDA: 44.26±10.25, 38.72±14.84, 31.59±12.68 vs 31.59±12.68; NO: 0.252±0.041, 0.223±0.037, 0.217±0.032 vs 0.331±0.092, P <0.05 or P <0.01), increased the significantly reduced level of expression of TGF-p and the activity of SOD in the model group (SOD: 30.16±2.88, 31.27±2.73, 33.52± 2.81 vs 28.33±1.17; TGF-β:0.136±0.031, 0.153±0.036, 0.169 ±0.029 vs 0.105±+0.021, P<0.05 or P<0.01), and also increased significantly the expression of EGF (EGF: 0.178±0.021, 0.195±0.031, 0.191±+0.022 vs 0.151±0.026, P<0.05 or P<0.01). The histological changes were also alleviated with ASP treatment. CONCLUSION: Enteroclysis with ASP markedly relieve the colon injury in acetic acid-induced rats colitis, which is related with promoting growth factors, decreasing oxygen free radicals and some anti-inflammation effects.

关 键 词：当归多糖 大鼠 乙酸性结肠炎 病理组织学 

分 类 号：R574.62[医药卫生—消化系统] R-332[医药卫生—内科学]