纤维粘连蛋白对培养的自发性高血压大鼠心肌成纤维细胞增殖及胶原合成的影响  

Effect of fibronectin on proliferation and collagen synthesis in cardiac fibroblasts from spontaneously hypertensive rats

作　　者：谢良地[1] 韩英[1] 陈水龙[1] 王华军[1] 许昌声[1] 

机构地区：[1]福建医科大学附属第一医院

出　　处：《中国病理生理杂志》2003年第12期1658-1661,共4页Chinese Journal of Pathophysiology

基　　金：高等学校骨干教师基金资助项目 (0 0A0 0 4 )

摘　　要：目的 :观察纤维粘连蛋白 (FN)对培养的SHR和WKY大鼠心肌成纤维细胞 (CFb ,CFbSHR,CFbWKY)增殖及胶原合成的影响。方法 :CFb取自 12周的SHR和WKY大鼠 ,采用组织块贴壁法培养 ,以直接细胞计数法和[3 H]-TdR掺入率反映细胞增殖 ,以 [3 H]-脯氨酸 ([3 H]-proline)掺入率反映胶原合成。使用FN(5 μg/cm2 )预先处理 2 4孔培养板。结果 :与 0 4 %FCS对照组相比 ,经 72h孵育FN明显促进CFbSHR和CFbWKY细胞数增多 ,分别为对照组的 16 3 75 % (CFbSHR)和 170 4 2 % (CFbWKY)。FN促进CFbSHR和CFbWKY[3 H]-TdR掺入增加 ,[CFbSHR:(44 734±8981)counts/minvs (2 92 33± 6 74 6 )counts/min ,P <0 0 5 ;CFbWKY:(314 6 1± 6 2 2 4 )counts/minvs (2 2 976± 4 782 )counts/min ,P <0 .0 5 ]。FN促进CFbSHR和CFbWKY[3 H]-proline掺入增加 [CFbSHR:(342 4± 114 5 )counts/minvs (1936± 76 3)counts/min ,P <0 .0 5 ;CFbWKY:(2 12 7± 4 15 )counts/minvs (15 32± 4 0 7)counts/min ,P <0 .0 5 ]。结论AIM: To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFb SHR ) and WKY (CFb WKY ). METHODS: CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and [ 3H]-TdR incorporation using 24-well plates pre-coated with 5 μg/cm 2 of FN. Collagen synthesis was determined by [ 3H]-proline incorporation. RESULTS: As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1×10 4 cells/mL. DNA synthesis of CFb was markedly promoted by FN [CFb SHR : ( 44?734 ± 8?981 )counts/min vs ( 29?233 ± 6?746 ) counts/min, P<0.05; CFb WKY : ( 31 461 ± 6 224 )counts/min vs ( 22 976 ± 4 782 ) counts/min, P<0.05 ]. FN induced an increased in [ 3H]-proline incorporation in both CFb SHR and CFb WKY [CFb SHR : ( 3 424 ± 1 145 )counts/min vs ( 1 936 ±763) counts/min, P<0.05; CFb WKY : ( 2 127 ±415)counts/min vs ( 1 532 ±407) counts/min, P<0 05 ]. CONCLUSION: FN is able to promote cell proliferation and collagen synthesis of CFb derived both from SHR and WKY.

关 键 词：心肌 成纤维细胞 纤连蛋白类 增殖 胶原 

分 类 号：R363[医药卫生—病理学]