食管癌BAT-26位点微卫星不稳的分析  

Analysis of microsatellite instability at BAT-26 locus in esophageal cancer

作　　者：颜坤[1] 王明荣[1] 房殿春[2] 杨康[1] 

机构地区：[1]第三军医大学附属西南医院胸心外科,重庆400038 [2]第三军医大学附属西南医院全军消化专科中心,重庆400038

出　　处：《第三军医大学学报》2003年第23期2101-2102,共2页Journal of Third Military Medical University

摘　　要：目的　探讨食管粘膜BAT 2 6位点微卫星不稳定性 (MicrosatelliteInstability ,MSI)及其单态性的情况。方法　组织DNA提取后PCR扩增 ,扩增产物行 9%变性聚丙烯酰胺凝胶电泳 ,最后银染等方法 ,分析 45例食管癌及正常切缘组织BAT 2 6位点微卫星不稳的情况。结果　所有正常组织在BAT 2 6位点DNA电泳条带长度无明显改变 ,45例食管癌中有3例MSI改变。结论　食管粘膜BAT 2 6具有较好的单态性 ,食管癌细胞BAT 2Objective To investigate the microsatellite instability(MSI) and monomorphism at microsatellite DNA BAT 26 locus in esophageal mucosa. Methods Genomic DNA extracted from tissues was amplified by PCR. PCR products were run on 9% denaturing polyacrylamidegel and stained with silver. Then MSI and monomorphism of microsatellite at BAT 26 locus in normal tissues at the incised edge and esophageal cancer tissues in 45 cases of esophageal caner were analyzed. Results All normal specimens showed no change in the length of MS DNA at BAT 26 locus. MSI was detected in 3 out of 45(6 7%) cases of esophageal cancer. Conclusion BAT 26 has complete monomorphism in genome of normal esophageal tissues, but is not sensitive to the identification of MSI.

关 键 词：微卫星 微卫星不稳定性 BAT-26 单态性 

分 类 号：R394.2[医药卫生—医学遗传学] R735.1[医药卫生—基础医学]