大鼠原发性结肠癌淋巴管的形态结构  

Morphologic Study of the Structures of the Lymphatics in Rat Primary Colonic Cancer

作　　者：马晶[1] 李益民[1] 聂春生[2] 赵玲辉[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学附属第三医院介入科,黑龙江哈尔滨150086

出　　处：《解剖科学进展》2004年第1期30-33,36,共5页Progress of Anatomical Sciences

基　　金：黑龙江省自然科学基金资助项目 (No D0 0 2 6;No D0 1 0 7)

摘　　要：目的　研究大鼠原发性结肠癌毛细淋巴管的微细分布、超微结构特征 ,观察毛细淋巴管的形态结构变化 ,为进一步探讨癌组织的淋巴道转移机制提供形态学依据。方法　取 4周龄大鼠 5 0只 ,用MNNG(甲基硝基亚硝其胍 )灌肠 ,建立大鼠结肠癌模型 ,分期取材 ,应用半薄切片光镜观察、超薄切片电镜观察的方法进行观察。结果　半薄切片观察可见癌组织中心区无毛细淋巴管和淋巴管 ;癌组织周边区毛细淋巴管数量增多 ,管腔扩张 ;电镜观察可见癌组织周边区毛细淋巴管内皮细胞发生溶解破坏 ,内皮细胞间开放连接增多 ,内皮细胞细胞器的形态发生明显改变。结论　癌细胞淋巴道转移可能是由于毛细淋巴管壁的破坏和内皮细胞的开放连接 。Objective To observe the morphology of lymphatics in the rat primary colonic cancer to provide the morphologic data for the study of the molecular mechanism of tumor metastasis. Method Wistar rats were used to establish the animal model of primary rat colonic cancer with MNNG to obtain the specimens in different stages. The lymphatics around the colonic cancer were observed with the methods of semithin section and electron-microscope. Results There were no lymphatics in the central region of carcinomas, but the density of the lymphatics was increased and the cavity of the lymphatics was dilated in the periphery region of cancer tissue. Under the electron-microscope, it was seen that the opening junction of lymphatic endothelial cells was widen and the damage of the lymphatic wall was found, the ultrastructure of lymphatic endothelium were changed in peripheral region of the cancer tissue. Conclusion The changes of the lymphatic endothelium in the periphery region of the colonic cancer tissue suggest that the cancer cells may enter lymphatic lumen through the opening of endothelial junction and the endothelial dissolution,the changes of the organelles of endothelial cell maybe related to the damage for lymphatic wall.

关 键 词：大鼠 原发性 结肠癌 淋巴管 形态结构 超微结构 

分 类 号：R735.35[医药卫生—肿瘤]