三氧化二砷诱导人鼻咽癌细胞凋亡的实验研究  

Experimental studies of apoptosis induced by arsenic trioxide in human nasopharyngeal carcinoma cells

作　　者：郑毓武[1] 林英城[1] 吴名耀[2] 李德锐[1] 吴贤英[2] 杜彩文[1] 陈炯玉[1] 洪超群[1] 

机构地区：[1]汕头大学医学院附属肿瘤医院内科,广东汕头515031 [2]汕头大学医学院病理学教研室,广东汕头515031

出　　处：《肿瘤防治杂志》2004年第1期35-37,共3页China Journal of Cancer Prevention and Treatment

基　　金：广东省医学科研课题 (A2 0 0 3 5 3 2 );汕头大学研究和发展基金项目 (L0 3 0 0 2 );汕头市重点科技计划项目 (汕府科[2 0 0 2 ] 90号 )

摘　　要：目的 :研究三氧化二砷 (As2 O3 )体外抑制人鼻咽癌细胞株 (CNE1)增殖及诱导凋亡的作用。方法 :以不同浓度的As2 O3 处理CNE1,用四甲基噻唑蓝 (MTT)法测定细胞增殖情况 ,细胞染色方法观察细胞凋亡的形态 ,原位末端标记法 (TUNEL)检测凋亡细胞 ,流式细胞仪检测细胞的DNA含量。结果 :As2 O3 明显抑制人鼻咽癌细胞株CNE1细胞的增殖 ,其抑瘤作用优于顺铂 (DDP)和 5 -氟尿嘧啶 (5 FU)。As2 O3 作用于细胞后 ,可见典型凋亡细胞的形态学改变 :细胞核固缩 ,染色质凝集 ,核碎裂 ,凋亡小体形成等。流式细胞仪DNA线条图上出现典型的亚二倍体“凋亡峰”。结论 :As2 O3OBJECTIVE:To investigate the effect of a wide range of concentrations of arsenic trioxide (As 2O 3) on human nasopharyngeal carcinoma cell strain in vitro .METHODS:CNE1 cell strains were dealt with As 2O 3 at different concentrations.Cell proliferation was evaluated by the MTT assay, and the cell apoptosis was evaluated by the cell morphology,flow cytometry and TUNEL methods.RESULTS:The cell proliferation was obviously depressed by As 2O 3 in vitro , which indicated that the depressive effect of As 2O 3 in cancer was better than DDP or 5 FU. The typical morphological features such as nuclear pycnosis, chromatin coagulation, nuclear fragmentation and apoptotic body formation were observed and a typical hypodiploid apoptosis peak in the DNA line was found in the flow cytometry.CONCLUSIONS:As 2O 3 can depress the proliferation of CNE1 and accelerate the apoptosis in vitro .

关 键 词：鼻咽肿瘤 病理学 砷剂 药理学 脱噬作用 药物作用 流式细胞术 

分 类 号：R739.63[医药卫生—肿瘤]