动态检测恶性腹水细胞凋亡的临床意义  

Clinical significance of dynamic measurement on apoptosis in malignant peritoneal effusion cells

作　　者：杨劲松[1] 张保国[1] 张学忠[1] 王书奎[2] 

机构地区：[1]南京医科大学附属南京第一医院肿瘤科,江苏南京210006 [2]南京医科大学附属南京第一医院放射免疫检测中心,江苏南京210006

出　　处：《肿瘤防治杂志》2004年第1期77-78,81,共3页China Journal of Cancer Prevention and Treatment

摘　　要：目的 :探讨检测恶性腹水细胞凋亡 (apoptosis ,APO)的价值及其与局部化疗疗效的关系。方法 :用流式细胞术的AnnexinV/PI双染色法检测外周血细胞以及恶性腹水局部化疗前后细胞APO水平。结果 :恶性肿瘤患者外周血APO [(4 92±3 0 7) % ]及腹水的APO [(1 81±1 2 5 ) % ]均高于正常对照组外周血[(1 70± 1 18) % ] ,但未经治疗的恶性腹水APO [(1 81± 1 2 5 ) % ]与正常对照组外周血相比 ,差异无统计学意义。治疗后恶性腹水APO高于治疗前 ,治疗有效及显效组APO[(8 2 9± 6 45 ) % ]与治疗前APO[(1 81± 1 2 5 ) % ]相比差异有统计学意义 ,P <0 0 1;且比局部化疗后无效组APO [(2 2 9± 2 0 3 ) % ]明显偏高 ,P <0 0 5。结论 :动态测定恶性腹水局部化疗前后APO的变化 ,在筛选药物、监测疗效及评估预后方面对临床有一定的指导意义。OBJECTIVES:To evaluate the value of measurement on apoptosis (APO) in malignant peritoneal effusion cells,and to study the relationship between APO and the therapeutic effect on intracavitary chemotherapy.METHODS:The flow cytometry’s Annexin V/PI method was used in measuring the APO level of peripheral blood cells and malignant peritoneal effusion cells before and after intracavitary chemotherapy.RESULTS:The APO rates of peripheral blood cells [(4 92±3 07)%] and peritoneal effusion cells [(1 81±1 25)%] in malignant tumor patients were both higher than the APO rate of peripheral blood cells [(1 70±1 18)%] in normal control group.However,there was no statistically significant difference between malignant peritoneal effusion [(1 81±1 25)%]in untreated patients and peripheral blood in normal control group in APO.The APO rate of malignant peritoneal effusion cells after treatment was higher than that before treatment.There was a more significant difference between them in the patients before treatment [(1 81± 1 25)%] and after treatment [(8 29±6 45)%] in effective or evidently effective group, P <0 01,as well as between them in effective or evidently effective group and ineffective group[(2 29±2 03)%], P <0 05.CONCLUSIONS:The dynamic analysis of the APO level of malignant peritoneal effusion cells before and after intracavitary chemotherapy not only helps to select effective drugs but also to evaluate the therapeutic effect and prognosis.

关 键 词：腹水 药物疗法 脱噬作用 流式细胞术 

分 类 号：R730.4[医药卫生—肿瘤]