乳腺浸润性导管癌微卫星不稳定性  

Microsatellite instability in invasive ductal carcinoma of the breast

作　　者：李志刚[1] 周晓军[1] 孔庆兖[2] 易龙[3] 孟奎[1] 

机构地区：[1]南京军区南京总医院病理科,南京210002 [2]徐州医学院病理学教研室,徐州221002 [3]南京大学医学院,南京210093

出　　处：《临床与实验病理学杂志》2004年第1期25-28,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的　探讨乳腺浸润性导管癌的微卫星不稳定 (MSI)性及其与临床病理资料的关系。方法　选取 10个微卫星位点 ,从石蜡包埋的存档标本中选取 34例肿瘤组织和其对应的自身正常对照组织 ,提取DNA后用PCR扩增 ,6 %聚丙稀酰胺凝胶电泳 ,银染显色后进行微卫星不稳定性分析。用免疫组化S P法观察 p5 3、c erbB 2、PR、ER在乳腺癌中表达情况。 结果　在 34例乳腺浸润性导管癌中有 9例 (2 6 4 7% )至少 1个位点出现MSI。MSI和病人年龄、肿瘤大小、病理分级、淋巴结转移、p5 3、c erbB 2之间没有明显的相关性。但ER和PR阴性的病例出现MSI的比例远远高于ER和PR阳性病例。 结论　在乳腺癌的发生、发展过程中出现MSI,并可能和ER。Purpose To investigate the microsatellite instability(MSI) in invasive ductal carcinoma of the breast and its relationship with clinicopathological parameters. Methods To analyzed 10 microsatellite loci in 34 paired breast cancer/normal tissue DNA samples. PCR products were electrophoresed on 6% polyacrylamide gel and detected using silver staining. The p53, c erbB 2,PR,ER status were determined by immunohistochemistry. Results Nine of 34 breast cancers(26 47%) showed evidence of MSI at least one locus. There was no significant association between MSI and age, histologic grade, tumor size, lymph node involvement, p53 and c erbB 2 status. A significant relationship was observed between patients with MSI tumors and negative ER and PR. Conclusions Breast cancer acquires MSI during tumorgenesis and may correlate with deregulation of ER and PR.

关 键 词：乳腺 浸润性导管癌 微卫星不稳定性 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]