免疫调节剂SE抗血吸虫性肝病变的CD_4^+细胞因子调节动态  

Dynamics of CD_4 ^+subclass cytokine regulation on hepatic granuloma in mice infected with Schistosoma japonicum induced by immunomodulator SE

作　　者：龚唯[1] 骆伟[1] 李允鹤[1] 夏超明[1] 周卫芳[1] 胡永德[1] 

机构地区：[1]苏州大学医学院寄生虫学教研室,苏州215007

出　　处：《中国人兽共患病杂志》2003年第4期75-77,共3页Chinese Journal of Zoonoses

基　　金：江苏省教育厅资助课题 (批号 :0 1KJD3 10 -0 0 1)4

摘　　要：目的　进一步研究免疫调节剂SE(含SE - 1和SE - 2 )对感染小鼠诱导的抗肝内血吸虫卵肉芽肿调节效应的动态变化和分子机制。方法　BALB/c小鼠 30只 ,均分 3组 ,2组分别用SE - 1和SE - 2皮下免疫共 3次 ,每次间隔 1w ,总剂量 10 5 μg和 16 0 μg/鼠 ,另为对照组。末次免疫后 10d ,3组每鼠攻击感染尾蚴 30± 2条。在感染后 7～ 11w时 ,剖杀小鼠 :取肝组织 ,制成石腊切片 ,观察和计算肉芽肿大小 ;无菌分离脾淋巴细胞 ,进行培养 ,取上清液测定IL - 2、IL - 4和IL - 10含量水平 (pg/ml)。 结果　小鼠感染后 7～ 11wk时 ,实验鼠肉芽肿的平均面积和体积均显著小于感染对照鼠 (P均 <0 0 1) ,实验组之间无显著性差异 (P均 >0 0 5 ) ;在感染后 7w时 ,实验鼠的IL - 2和IL - 4水平均显著高于对照鼠 ;3组均显示相当高水平的IL - 10 ;在感染后 11w时 ,实验鼠的IL - 10水平显著高于对照鼠 (P均 <0 0 5 )。结论　免疫调节剂SE能诱导明显的抗肝内血吸虫卵肉芽肿效应和血吸虫性纤维化作用 ,其分子机制与Th1型 (IL - 2 )和Th2型 (IL - 4和IL - 10 )细胞因子应答的调节动态密切相关。Aim In order to further investigate the regulatory effects and molecular mechanisms of hepatic granuloma in mice infected with Schistosoma japonicum induced by immunomodulators(including SE 1 and SE 2) Methods Thirty mice were divided evenly into 3 groups,mice in both groups were immunized subcutaneously with SE 1 and SE 2 three times,an interval of one week between two times,the total dosage was 105μg and 160μg per mouse,respectively Ten days after stopping immunization,mice were killed for observations of different indices during 7th to 11th week after infection Results The mean area and volume of hepatic egg granuloma in group SE 1 and group SE 2 were more significantly decreased respectively than those in control group(P<0 01)at 7wk,9wk and 11wk post infection,the size of hepatic egg granuloma had no significant difference(P>0 05)between group SE 1 and group SE 2 The IL 2 and IL 4 levels(pg/ml)in both groups were obviously higher than those in control group at 7wk post infection,and the IL 10 contents of above mentioned three groups were detected more higher levels; the IL 10 levels of two experimental groups were obviously higher(P<0 05)than that in control group at 11wk after infection Conclusion Immunomodulator SE could induce markedly regulatory effects of anti hepatic egg granuloma and schistosome fibrosis in infected mice,the molecular mechanisms are closely associated with the dynamics of regulatory effects of Th1 type(IL 2)and Th2 type(IL 4 and IL 10)cytokines responses

关 键 词：免疫调节剂 SE 抗血吸虫性肝病变 CD4^+ 细胞因子 调节 日本血吸虫 

分 类 号：R383.24[医药卫生—医学寄生虫学]