应用光动力学方法制作实验性视网膜静脉阻塞模型  

Experimental retinal vein occlusion induced by photodynamic approach

作　　者：张晓丽[1] 胡运韬[1] 马志中[1] 王常观[1] 韩素义 杨炳健[1] 黄丽洁[2] 

机构地区：[1]解放军总医院眼科 [2]解放军总医院实验动物中心,北京100853

出　　处：《中华眼科杂志》2003年第4期220-223,共4页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 ( 3 9870 798)

摘　　要：目的　应用光动力学方法制备实验性视网膜静脉阻塞模型 ,以观察眼底形态学和病理学改变。方法　取小型猪 15只 ,随机取单眼为实验组 ,静脉推入孟加拉玫红 (2 0mg/kg) ,1min后采用玻璃体切割机眼内照明的导光纤维直接照射视盘上方 0 5～ 1 0PD的视网膜主干静脉 15min ;分别于血栓形成后 1h、3d、7d、14d、2 1d及 2 8d进行间接眼底镜观察、荧光素眼底血管造影检查 ,取静脉阻塞区视网膜及血管行光镜和透射电子显微镜观察。对照组对侧眼不注射孟加拉玫红 ,直接用光导纤维照射靶血管 2 0min ,取出光导纤维 ,缝合切口。分别于光导纤维照射后 1h、2 4h、3d后观察眼底改变 ,同时摘除眼球 ,行光镜及电镜观察。结果　视网膜静脉血栓形成可靠 ,可见视网膜静脉纡曲扩张、广泛出血及水肿等典型病理改变。结论　利用导光纤维眼内照明技术与化学光敏剂方法制作的实验性视网膜静脉阻塞模型 ,是一种操作简单、定位准确、血栓形成可靠的实验技术。Objective To develop a new photochemical method of experimental retinal vein occlusion and investigate the morphologic and histologic changes in the retina. Methods After intravenous injection of rose Bengal, the vessels next to the disc of miniature pigs in the experiment group ( n =15) were exposed to an endo illuminator for 15 minutes. As a control group, the vessels next to the disc of each pig were exposed to the endo illuminator without rose Bengal injection. After complete vascular occlusion, the eyes were observed at following times: one hour, and 1, 3, 7, 14, 21, and 28 days. After the 28th day, the eyes were enucleated and prepared for light and electron microscope examination. Results Histopathologic features were consistent with changes of retinal vein occlusion and formation of retinal vein thrombi was reliable. Conclusions Photodynamic method combined with endo illuminator was a simple, reliable and definitive experimental technique to produce retinal vein occlusion.

关 键 词：光动力学 制作技术 实验性视网膜 静脉阻塞模型 

分 类 号：R774.1[医药卫生—眼科]