Changes of metabotropic glutamate receptor subtype 1a in diffuse brain injury with secondary brain insults and the effects of 2-methyl-4-carboxyphenylglycine  

作　　者：费舟 章翔 刘恩渝 

机构地区：[1]Department of Neurosurgery,Xijing Hospital,Fourth Military Medical University

出　　处：《Chinese Journal of Traumatology》2003年第5期270-274,共5页中华创伤杂志（英文版）

基　　金：NSFC (30 2 70 5 34);FoundationforUniversityKeyTeacherbytheMinistryofEducation

摘　　要：Objective: To observe the changes of metabotropic glutamate receptor 1a in rat brain in a rodent model of diffuse head injury with secondary insults and the effects of 2 methyl 4 carboxyphenylglycine (MCPG). Methods: Based on Marmarous rodent model of diffuse brain injury (DBI), hypotension was made by blood withdrawal as secondary brain insults (SBI). 105 male SD rats were randomized into A and B groups. The changes of mGluR 1a in cerebral cortex were studied by immunohistochemistry and the effect of MCPG by HE. Each group was divided into different subgroups at different time after injury. Results: Compared with that of sham group, the number of mGluR 1a positive neuron increased by 12.9±3.2 (P< 0.05 ) 1 day after injury in the injured cerebral cortex in DBI group. However, in DBI and SBI group there was a more significant increase in the number of mGluR 1a positive neuron at 4 hours after injury ( 15.6±3.0 , P< 0.05 )and then the number of mGluR 1a positive neuron gradually decreased. Administration of MCPG reduced total cortical necrotic neurons counts on the 7th day after injury ( 5.21±2.52 , P< 0.05 ). Conclusions: Brain injury can increase the gene expression of mGluR 1a and the role of mGluR 1a may be a key factor in the aggravation of head injury with SBI, and that MCPG may have therapeutic potential in head injury.

关 键 词：Brain injuries Receptors  metabotropic glutamata 

分 类 号：R741[医药卫生—神经病学与精神病学]