Effect of estradiol on chemokine receptor CXCR2 expression in rats: implications for atherosclerosis  

雌激素对大鼠趋化因子受体CXCR2表达的影响:动脉粥样硬化的指征(英文)

作　　者：雷著斌[1] 付绪杰[1] 卢兆桐[1] 王宝成[1] 刘现亮[1] 尤乃桢[1] 

机构地区：[1]济南军区总医院心内科

出　　处：《Acta Pharmacologica Sinica》2003年第7期670-674,725,共6页中国药理学报（英文版）

基　　金：Project supported by the Medicinal Foundation of the Chinese People's Liberation Army (01Q015).

摘　　要：AIM: To study the effect of 17β-estradiol on expression of chemokine receptor CXCR2 in monocytes in vivo. METHODS: Expressions of chemokine receptor CXCR2 mRNA and protein were measured by RT-PCR and flow cytometry, respectively. RESULTS' In both ovary-intact and ovariectomized (OVX)rats, CXCR2 protein and mRNA expression were significantly increased in rats fed with a high-cholesterol diet for 6 weeks. The cholesterolinduced increases in CXCR2 protein and mRNA expression were significantly attenuated in OVX rats injected with estradiol-17β (17β-E_2) (5 and 20 μg·kg^(-1)·d^(-1)). In normal diet rats, CXCR2 protein and mRNA expression were increased in OVX rats compared with ovary-intact rats, and this increase was prevented by 17β-E_2. CONCLUSION: Both basal and hypercholesterolemia-induced increases in chemokine receptor CXCR2 are modulated by physiological concentrations of estradiol.目的:研究17β-雌二醇对体内单核细胞趋化因子受体CXCR2表达的影响。方法:流式细胞仪测定单核细胞趋化因子受体CXCR2的蛋白表达、逆转录PCR测定其mRNA水平。结果:在卵巢完整和切除卵巢的大鼠中,喂养富含胆固醇饮食6周增加单核细胞趋化因子受体CXCR2 mRNA和蛋白水平,在切除卵巢的大鼠,注射17β-雌二醇(5和20μg·kg^(-1)·d^(-1))显著减少了胆固醇诱导的CXCR2 mRNA及蛋白表达的增加,正常饮食的大鼠,切除卵巢增加了趋化因子受体CXCR2 mRNA和蛋白水平的表达,但是注射17β-雌二醇可以防止它表达的增加。结论:生理性浓度的雌激素调节基础及胆固醇诱导的趋化因子受体CXCR2表达。

关 键 词：17Β-ESTRADIOL chemokine receptors MONOCYTES flow cytometry reverse transcriptase polymerase chain reaction 

分 类 号：R96[医药卫生—药理学]