人类高分辨染色体原位杂交及βHCG基因定位的研究  

A STUDY ON HUMAN HIGH RESOLUTION CHROMOSOMAL LOCALIZATION OF HUMAN GENE FOR βHCG BY IN SITU HYBRIDIZATION

作　　者：刘希贤[1] 虞京葳 

机构地区：[1]同济医科大学医学遗传研究室

出　　处：《中华医学遗传学杂志》1992年第6期345-347,共3页Chinese Journal of Medical Genetics

摘　　要：应用一种原位杂交后G显带的方法,把人类绒毛膜促性腺激素β亚基(βHCG)基因定位于11q21→qter。此外,对DNA片段探针与含探针DNA片段的重组体质粒在原位杂交的效果作了比较,两者无显著差异。An improved method for G banding chromosomes after in situ hybridization is reported here. Using this procedure, we assigned the specific chromosome site, the β-subunit of human chorionic gonadotropin (βHCG) gene to 11q21→qter in a male peripheral lymphocyte sample. Besides, the effects of in situ hybridization with probe DNA fragment and that with recombinant plasmid containing an insert probe DNA were compared.

关 键 词：杂交 高分辨 染色体 重组 基因 

分 类 号：R394.8[医药卫生—医学遗传学]