脑出血后脑组织内凝血酶受体1(PAR1)的表达及其病理意义  

Role of thrombin receptor in the pathogenesis of cerebral hemorrhage in rats

作　　者：姜亚军[1] 常诚[1] 张琳[2] 吴明华[1] 符为民[1] 吴灏昕[3] 

机构地区：[1]南京中医药大学附属江苏省中医院脑病中心,江苏南京210029 [2]东南大学附属中大医院神经内科,江苏南京210009 [3]南京中医药大学基础医学院,江苏南京210029

出　　处：《中风与神经疾病杂志》2004年第2期100-103,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金 (3 0 171166)

摘　　要：目的　探讨脑出血后脑组织内凝血酶受体 1(PAR1)的表达规律及其意义。方法　成年大鼠分为 3组 ,分别向大脑基底节区注入全血、全血加水蛭素、或生理盐水。于注射后 6 h、2 4 h、72 h和 7d,取脑组织 ,检测脑内PAR1表达 (免疫组织化学法 )。同时 ,观察神经细胞的凋亡情况 (Tunel法 )和脑组织水含量 (干燥法 )。结果　与生理盐水对照组比较 ,脑出血后血肿周围脑组织内 PAR1免疫阳性细胞数明显升高 (P<0 .0 5 )。同时 ,脑组织内凋亡细胞数和脑组织水含量也明显增加。时效学研究显示 ,PAR1表达上调开始于脑出血后 6 h,72 h达高峰 ,7d后下降。加用凝血酶抑制剂 -水蛭素者 ,出血周围组织内 PAR1表达明显抑制 ,同时脑组织水肿和神经凋亡显著减轻。结论　脑出血后脑组织 PAR1表达增加。 PAR1高表达可能介导了脑出血后神经损伤的过程。Objective To investigate the expression of thrombin receptor 1 (PAR1) and its role in the pathogenesis of intracerebral hemorrhage (ICH).Methods The adult rats were divided into three groups,and intracerebrally given with autologous whole blood (50μl),autologous blood plus hirudin thrombin solution (10 units),or normal saline (NS),respectively.At 6,24,72 hours and 7days after injection, the brains were taken out for detecting the PAR1 expression (Immunostaining method) and the apoptosis cell (Tunel method).The water content of brains was also assayed.Results The number of positive PAR1-immunostaining cells in the tissue surrounding hemotoma were markedly increased at 6h after ICH,peaked at 72h and decreased at 7d after ICH.At the same time,the apoptosis cells and brain water contents were also increased. However PAR1 expression, apoptotic cells and brain water content were markedly inhibited in cases of coinjection of Hirudin and blood.Conclusion PAR1 expression was upregulated during ICH.The activation of PAR1 might be involved in the pathogenesis of ICH.

关 键 词：脑出血 后脑组织 凝血酶受体 PAR1 组织表达 病理分析 

分 类 号：R743.34[医药卫生—神经病学与精神病学]