新生隐球菌Cap59缺陷株ura5突变株的筛选方法  

SELECTION FOR URA5 MUTANTS OF CRYPTOCCOCUS NEOFORMANS CAPSULE-DEFICIENT STRAIN CAP59

作　　者：郭秀军[1] 廖万清[1] 任大明[1] 王荫榆[1] 

机构地区：[1]北京解放军306医院皮肤科,北京100101

出　　处：《微生物学通报》2004年第2期105-107,共3页Microbiology China

基　　金：国家自然科学基金资助项目 (No 395 70 0 4 3)

摘　　要：改进筛选新生隐球菌Cap5 9荚膜缺陷株ura5突变株的方法。采用硫酸二乙酯化学诱导新生隐球菌Cap5 9荚膜缺陷株 ,利用 5 氟乳清酸 (5 FOA)反筛选法筛选ura5尿嘧啶合成基因突变株。用新方法筛选到 2株Cap5 9荚膜缺陷株ura5突变株。This study was to improve the way for selecting ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strains.They were induced by Diethyl Sulfate. Ura5 mutants were screened by 5-fluoroorotic acid counter selection method. Using the new method, we obtained two ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strain.A easy method that was used to screen ura5 mutants of Cryptoccocus neoformans has been established.

关 键 词：新生隐球菌 ura5突变株 

分 类 号：Q933[生物学—微生物学]