人尿中一种抗菌物质三甲氧苄二氨基嘧啶的分离、纯化和鉴定  

Separation,Purification and Identification of Trimethoprim——an Antibiotic from Male Urine

作　　者：李强[1] 陈钧辉[1] 张冬梅[1] 包燕雏[2] 张海燕[1] 麻亚锋[1] 王新昌[1] 

机构地区：[1]南京大学生物化学系医药生物技术国家重点实验室,南京210093 [2]南京大学现代分析中心,南京210093

出　　处：《中国生物化学与分子生物学报》2004年第2期280-282,共3页Chinese Journal of Biochemistry and Molecular Biology

基　　金：江苏省自然科学基金资助项目 (No .BK970 3 6)~~

摘　　要：In order to explain the special antibiotic activity of urine, various chromatographic techniques were applied to separate and purify the key component from the healthy male urine. The structure of purified component was identified by mass spectrometry and NMR. The results indicated that the substance separated from the male urine was trimethoprim. This is the first report of trimethoprim separated from urine.In order to explain the special antibiotic activity of urine, various chromatographic techniques were applied to separate and purify the key component from the healthy male urine. The structure of purified component was identified by mass spectrometry and NMR. The results indicated that the substance separated from the male urine was trimethoprim. This is the first report of trimethoprim separated from urine.

关 键 词：人尿 抗菌物质 三甲氧苄二氨基嘧啶 分离 纯化 鉴定 二氢叶酸还原酶 抑制剂 化学药物 细菌感染 

分 类 号：R978[医药卫生—药品]