胃癌变前后凋亡相关蛋白和PCNA的表达  

Expression of apoptosis-related proteins and proliferating cell nuclear antigen during stomach canceration

作　　者：伍银桥[1] 王孟薇[1] 吴本俨[1] 尤纬缔[1] 祝庆孚[2] 

机构地区：[1]中国人民解放军总医院南楼消化科,北京市100853 [2]中国人民解放军总医院病理科,北京市100853

出　　处：《世界华人消化杂志》2004年第4期770-773,共4页World Chinese Journal of Digestology

基　　金：军队"十五"重点科研基金资助项目;No.01Z035~~

摘　　要：目的:探讨凋亡相关基因p53,Bcl-2和增生细胞核抗原(PCNA)在胃癌发生中的作用以及在胃癌早期诊断中的意义. 方法:采用免疫组化(LSAB)方法检测52例胃癌癌变前后组织标本的p53,Bcl-2和PCNA的表达. 结果:肠上皮化生、不典型增生和胃癌的p53蛋白表达分别为27.8%,38.2%和57.7%;Bcl-2蛋白表达分别为33.3%, 50.0%和65.4%;PCNA LI分别为41.4±13.0,47.9±8.9和53.0±11.9.在肠型胃癌,癌前p53,Bcl-2阳性者发展到癌时仍全为阳性,无1例转阴性;在弥漫型胃癌,癌前Bcl-2阳性者到癌时有1例转阴;PCNA LI由癌前发展到癌时明显增高,在肠型胃癌差异有显著性(P<0.001). 结论:在肠型胃癌发生发展过程中,p53,Bcl-2蛋白表达起重要作用,且细胞增生活性逐渐升高.AIM: To study the role of apoptosis-related gene p53, Bcl-2, and proliferating cell nuclear antigen (PCNA) in gastric cancinogenesis. METHODS: Immunohistochemical method (LSAB) was used to assess p53, Bcl-2, PCNA expression in specimens of 52 patients with gastric cancer and precancerous lesions. RESULTS: In the patients with intestinal metaplasia, dys-plasia and gastric cancer, the expression rates of p53 were 27.8%, 38.2% and 57.7% respectively. The expression rates of Bcl-2 were 33.3%, 50.0% and 65.4%, and PCNA indexes were 41.4±13.0, 47.9±8.9 and 53.0±11.9 respectively. In patients with intestinal-type carcinoma, p53 and Bcl-2 remained expressed in all precancerous tissues after development into cancer. In diffuse-type carcinoma, the positive Bcl-2 expression became negative in one patient. PCNA LI markedly increased from precancerous lesions to cancer, and a significant difference was observed in intestinal-type carcinoma (P <0.001). CONCLUSION: The expression of p53 and Bcl-2 significantly influences the development of intestinal-type gastric carcinoma, and the proliferating activity of the cancer cells gradually increase.

关 键 词：胃癌 PCNA 基因表达 细胞调亡 肿瘤 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]