蛋白质组中蛋白质磷酸化研究进展  

Recent Advances of Protein Phosphorylation in Proteome

作　　者：杨策[1] 王正国[1] 朱佩芳[1] 

机构地区：[1]第三军医大学野战外科研究所,重庆400042

出　　处：《生理科学进展》2004年第2期119-124,共6页Progress in Physiological Sciences

基　　金：国家重点基础研究发展规划项目 (G19990 5 4 2 0 0 )资助课题

摘　　要：随着后基因组时代的到来 ,对生命体器官、组织或细胞的全部蛋白质的表达、修饰及相互作用的研究已成为蛋白质组学的重要任务。蛋白质磷酸化是细胞内信号转导和酶调控最常见的机制之一 ,人类基因组约 2 %的基因编码 5 0 0种激酶和 10 0种磷酸酶。蛋白质磷酸化和去磷酸化作为原核和真核细胞表达调控的关键环节 ,了解其对功能的影响可以深入理解生命系统在分子水平的调控状况。目前蛋白质组磷酸化研究仍是功能基因组面临的重大课题 。With the advent of post-genomic era, it is an important task to study the expression, modification and interaction of the total proteins in cells, tissue and organs for proteomics in biological organism. The protein phosphorylation is one of the most popular mechanisms in signal transduction and enzyme modulation. It is estimated that about 2% of the total genes encoded 500 kinases and 100 phosphatases in human genome. As the key point of expression modulation in prokaryotic and eukaryotic cells, the protein phosphorylation and dephosphorylation may help to reveal the status of the life system at the molecular level. The phosphorylation in proteome remains challenging for functional genomics. In the present review, some new advances such as identification and characterization of protein phosphorylation in proteome are summarized.

关 键 词：蛋白质组 蛋白质 磷酸化 二维电泳 信号转导 质谱 

分 类 号：Q51[生物学—生物化学]