Dot-PPA-ELISA检测猪血清伪狂犬病抗体的研究  

Studies on the detection of PRV antibody using Dot-PPA-ELISA in pig serum

作　　者：曹三杰[1] 文心田[1] 

机构地区：[1]四川农业大学动物科技学院预防医学系,四川雅安625014

出　　处：《中国预防兽医学报》2004年第3期213-215,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：本研究建立了Dot＿PPA＿ELISA检测猪伪狂犬病抗体的方法。制备的诊断膜片PRV点样抗原含量为0 5μg,可以检测的最低猪IgG含量为1 398×10＿8g,灵敏性高。不与猪瘟、猪细小病毒病、猪衣原体病、布氏杆菌病、日本乙型脑炎、口蹄疫阳性血清发生交叉反应,特异性好。研究初步确定检测猪伪狂犬病抗体的阳性标准为血清效价1∶64以上。试验结果表明:该法具有操作方便、快速,结果客观,易于判读等优点,可应用于猪场伪狂犬病的诊断和抗体检测。A Dot-PPA-ELISA was developed for detecting the pig serum antibodies of PRV.There are 0.5μg antigen of PRV fixed on every little diagnostic membrane. The results of test indicated that Dot-PPA-ELISA is a very sensitive and specific method to detect the PRV serum antibody. It can be detected that the minimum IgG of swine is 1.398×10^(-8)g. There are no cross reaction with the positive pig serum of HCV , PPV, CHL, Brucella, JEV, and FMDV. If the serum titre is 1∶64 or over that using this way to detect the pig serum of PRV, the detect results are positive. There are many advantages of Dot-PPA-ELISA , it is easy and rapid to detect antibody and the results is very easy to read etc. This method could detect or diagnose the PRV disease in pig farm.

关 键 词：DOT-PPA-ELISA 伪狂犬病病毒 抗体 

分 类 号：S852.5[农业科学—基础兽医学] S852.65[农业科学—兽医学]