RP-HPLC法测定口服麦胚凝集素修饰脂质体中胰岛素  

RP-HPLC determination of insulin in WGA modified insulin liposomes

作　　者：张娜[1] 周玲[2] 徐文方[3] 袁玉梅[3] 李银萍[1] 

机构地区：[1]山东大学药学院药剂学教研室,山东济南250012 [2]济南市妇幼保健院药剂科,山东济南250001 [3]山东大学药学院药物化学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2004年第2期232-234,共3页Journal of Shandong University:Health Sciences

基　　金：山东大学青年基金(21310053187056〕

摘　　要：目的:建立RP-HPLC方法,测定麦胚凝集素修饰脂质体中胰岛素的含量和包封率。方法:利用逆相蒸发-超声法制备麦胚凝集素修饰胰岛素脂质体,反相高效液相色谱法测定脂质体中胰岛素含量和包封率。利用Phenomenex-C_(18)柱(250mm×4.6mm,5μm),乙腈-0.01％三氟乙酸(300:700)为流动相,测定波长为210nm,建立了HPLC测定胰岛素含量的方法。结果:HPLC测定方法线性范围5～100μg/ml,线性关系良好。普通胰岛素脂质体的包封率为(30.30±2.77)％(n=3),麦胚凝集素修饰脂质体包封率为(69.33±4.54〕％(n=3)。结论:建立的RP-HPLC方法便捷准确,重现性好,可用于测定麦胚凝集素修饰脂质体中胰岛素的含量和包封率。Objective:To develop a RP-HPLC method for determining the contents of insulin in wheat germ agglutinin(WGA)modified insulin liposomes and the entrapment efficiencies of the liposomes. Methods:Insulin-liposomes were prepared by reversed-phase evaporation and sonication.Thh entrapment efficiencies of the liposomes and the contents of insulin in the liposomes were analyzed by RP-HPLC. Chromatographic conditions included Phenomenex-C_(18)(25 mm×4.6 mm,5 μm)and mobile phase consisting of a mixture of acetonitrile-acetate trifluoride (0.01%).Detection wavelength was 210 nm.Results:The calibration curve was linear in the range of 5～100μg/ml.The entrapment efficiencies of conventional li- posome and WGA modified insulin liposomes were(30.30±2.77)%(n=3)and (69.33±4.54)%(n=3),respec- tively.Conclusion:The method is convenient,accurate,reproducible,and suitable for determining the contents of insulin in WGA modified insulin liposomes and the entrapment efficiencics of the liposomes.

关 键 词：反相高效液相色谱法 胰岛素脂质体 麦胚凝集素 包封率 

分 类 号：R945[医药卫生—微生物与生化药学]