幽门螺杆菌相关性胃疾病血清IL-8和NO含量的检测及意义  

Signifecance of serum level of NO and IL-8 in helicobacter pylori associated gastric diseases

作　　者：宋春芳[1] 孙丽萍[1] 戴文颖[1] 袁媛[1] 

机构地区：[1]中国医科大学第一附属医院肿瘤研究所,沈阳110001

出　　处：《中华肿瘤杂志》2003年第3期258-260,共3页Chinese Journal of Oncology

基　　金：国家重点基础研究发展规划"973"项目 (G19980 5 12 0 3 )

摘　　要：目的　检测幽门螺杆菌 (HP)相关性胃疾病患者血清白细胞介素 8(IL 8)和NO浓度 ,探讨其与HP感染的关系 ,以及HP感染引起胃上皮细胞增殖与凋亡失衡 ,导致胃癌形成的可能分子机制。方法　以ELISA法检测血清IL 8浓度 ,镀铜镉粒还原法检测血清NO浓度。结果　IL 8浓度在正常组织 (2 2 .5 0± 1.87pg ml)、浅表性胃炎 (34.99± 7.89pg ml)、萎缩性胃炎 (6 5 .2 7± 10 .6 0pg ml)及胃癌 (94 .84± 11.0 9pg ml)组间差异有显著性 (P <0 .0 1) ;萎缩性胃炎组NO浓度 (39.93± 5 .4 3μmol L)明显高于胃癌组 (37.0 2± 4 .13μmol L ,P <0 .0 5 ) ,其余各组间差异无显著性。HP(+)组IL 8和NO浓度显著高于HP(- )组 (77.30± 2 0 .92pg ml,39.16± 14 .4 0pg ml,P <0 .0 1;39.77± 5 .5 7μmol L ,35 .35±5 .2 4 μmol L ,P <0 .0 1) ;CagA(+)HP组IL 8和NO浓度显著高于HP(- )组 (83.4 5± 16 .92pg ml,6 6 .2 4± 2 3.2 1pg ml,P <0 .0 1;4 0 .97± 4 .5 9μmol L ,37.6 2± 6 .5 8μmol L ,P <0 .0 5 )。浅表性胃炎及萎缩性胃炎组的IL 8与NO呈正相关 (r分别为 0 .881和 0 .995 ) ,正常组和胃癌组无相关性。结论　血清IL 8和NO浓度与CagA(+)HP菌株感染密切相关 ;血清IL 8和NO浓度测定与HP菌株CagA分型联合检测将有助?Objective To investigate the relation between serum level of interleukin 8(IL 8)?nitrogen monoxide(NO) and helicobacter pylori(HP) infection, as well as the possible molecular mechanism of HP infection causing the imbalance of apoptosis and proliferation in gastric epithelial cells, which may lead to oncogenesis in stomach. Methods Serum IL 8 level was detected with enzyme linked immunosorbent assay (ELISA). Serum NO was detected with chrome reduction method using plated copper. Results Serum level of IL 8 were 22.50±1.87 pg/ml in the normal tissue, 34.99± 7.89 pg/ml in superficial gastritis, 65.27±10.60 pg/ml in atrophic gastritis and 94.84±11.09 pg/ml in gastric cancer ( P <0.01). Serum level of NO in the atrophic gastritis group(39.93±5.43 μmol/L)was significantly higher than that in the gastric cancer group(37.02±4.13 μmol/L)( P < 0.05 ). The differences in the other groups were not significant. IL 8 and NO levels in the HP(+)group were significantly higher than those in the HP(-) group(77.30±20.92 pg/ml vs 39.16±14.40 pg/ml, P <0.01; 39.77±5.57 μmol/L vs 35.35± 5.24 μmol/L, P <0.01). Serum levels of IL 8 and NO in the cytotoxin associated gene A protein (CagA)(+)HP group were significantly higher than those in the CagA(-)HP group(83.45±16.92 pg/ml vs 66.24±23.21 pg/ml, P <0.01; 40.97±4.59 μmol/L vs 37.62±6.58 μmol/L, P < 0.05). Serum levels of IL 8 and NO showed positive correlation between superficial gastritis and atrophic gastritis groups ( r =0.881, r =0.995), whereas no correlation was found in the normal or gastric cancer groups. Conclusion Serum levels of IL 8 and NO are correlated with CagA(+)HP strain infection. Combined detection of serum level of IL 8?NO and HP CagA will contribute to the early diagnosis of precancerous lesion in the stomach. [

关 键 词：幽门螺杆菌 胃疾病 白细胞介素-8 一氧化氮 胃癌前病变 上皮细胞 

分 类 号：R735.2[医药卫生—肿瘤]