Apoptosis of human gastric adenocarcinoma cells induced by β-ionone  

作　　者：Jia-RenLiu 3ing-QingChen Bao-FengYang Hong-WeiDong Chang-HaoSun Qiwang GuoSong You-Qiangsong 

机构地区：[1]PublicHealthCollege,HarbinMedicalUniversity,Harbin150001,HeilongjiangProvince,China [2]DepartmentofPharmacology,HarbinMedicalUniversity,Harbin150086,HeilongjiangProvinceChina [3]DepartmentofBiochemistryandTheGenomeResearchCenter,TheUniversityofHongKong,HongKong,China

出　　处：《World Journal of Gastroenterology》2004年第3期348-351,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by The National Natural Science Foundation of China,No.30200229 and The Postdoctoral Foundations of China and Heilongjiang Province,China

摘　　要：AIM:To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.METHODS: Using M-IT, fluorescence dye (Hoechst-33258),transmission electron microscopy and the TUNEL assay,we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200μmol/L) for 24h,48h.RESULTS:The growth of SGC-7901 cells was inhibited by β-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%,78.25% and 94.15%, respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89μmol/L.The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-iononetreated SGC-7901 cells by the TUNEL assay.CONCLUSION:β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells.However, the mechanism needs to be further investigated.AIM:To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901. METHODS:Using MTT,fluorescence dye(Hoechst-33258), transmission electron microscopy and the TUNEL assay,we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations(i.e.25,50,100 and 200μmol/L)for 24h,48h. RESULTS:The growth of SGC-7901 cells was inhibited by β-ionone.Seven days after treatment with β-ionone at four concentrations,the inhibition rates were 12.04%,30.59%, 78.25% and 94.15%,respectively.The IC_(50) value of β-ionone for SGC-7901 cells was estimated to be 89μmol/L.The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy.Apoptosis was also shown in β-ionone- treated SGC-7901 cells by the TUNEL assay. CONCLUSION:β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells.However,the mechanism needs to be further investigated.

关 键 词：β-紫罗酮 胃腺癌 肿瘤细胞 细胞凋亡 TUNEL法 发射电子显微镜 肿瘤抑制 

分 类 号：R735.2[医药卫生—肿瘤]