Gonadectomy affects brain derived neurotrophic factor in rats after chronic constriction nerve injury  

作　　者：XinZHAO XinWANG Shu-yunZHENG Jian-guoXU 

机构地区：[1]SchoolofLifeScience,NanjingUniversity,Nanjing210093 [2]SchoolofLifeScience,NanjingUniversity,Nanjing210093 [3]DepartmentofAnesthesiology,JinlingHospital,Nanjing210002,China

出　　处：《Acta Pharmacologica Sinica》2004年第3期286-292,共7页中国药理学报（英文版）

摘　　要：AIM: To assess the effect of gonadectomy on brain derived neurotrophic factor (BDNF) expression in neuropathic pain. METHODS: Using chronic constriction injury (CCI) model, we detected BDNF mRNA in dorsal root gan- glion and protein content in spinal cord by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay respectively. The time point we chose was post CCI operation d 0, 3, 7, 14, and 21. RESULTS: After CCI surgery, BDNF mRNA in ipsilateral DRGs was upregulated and reached its maximum on post operation d 7. BDNF protein level in ipsilateral spinal cord was also increased and reached its maximum on post operation d 14. The magnitude of this increase in gonadectomy (GDX) rats was significantly smaller than the GDX-sham rats at each time point. CONCLUSION: Gonadectomy reduced the BDNF increment after CCI surgery. Estrogen may affect nociceptive processing by its effect on BDNF.AIM: To assess the effect of gonadectomy on brain derived neurotrophic factor (BDNF) expression in neuropathic pain. METHODS: Using chronic constriction injury (CCI) model, we detected BDNF mRNA in dorsal root gan- glion and protein content in spinal cord by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay respectively. The time point we chose was post CCI operation d 0, 3, 7, 14, and 21. RESULTS: After CCI surgery, BDNF mRNA in ipsilateral DRGs was upregulated and reached its maximum on post operation d 7. BDNF protein level in ipsilateral spinal cord was also increased and reached its maximum on post operation d 14. The magnitude of this increase in gonadectomy (GDX) rats was significantly smaller than the GDX-sham rats at each time point. CONCLUSION: Gonadectomy reduced the BDNF increment after CCI surgery. Estrogen may affect nociceptive processing by its effect on BDNF.

关 键 词：GONADECTOMY ESTROGENS brain-derived neurotrophic factor gene expression regulation PAIN chronic constriction injury 

分 类 号：R651[医药卫生—外科学]