Identification of genes responsive to apoptosis in HL-60 cells  

作　　者：WeiJIN Le-fengQU PingMIN ShanCHEN HongLI HeLU Yong-taiHOU 

机构地区：[1]ShanghaiInstituteofMateriaMedica,ChineseAcademyofSciences,Shanghai201203 [2]DepartmentofVascularSurgery＆InstituteofVascularSurgeryofPLA,ChanghaiHospital,Shanghai200433 [3]Lepblefranco-chinoisduscienceduvivantetgenomique,RuijinHospital,Shanghai200025,China [4]CorrespondencetoDrYong-taiHOU

出　　处：《Acta Pharmacologica Sinica》2004年第3期319-326,共8页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundationof China (30200151) and One Hundred People Programs ofChinese Academy of Sciences (2001).

摘　　要：AIM: To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine. METHODS: cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis. RESULTS: Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways. CONCLUSION: TGFβ and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis.AIM: To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine. METHODS: cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis. RESULTS: Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways. CONCLUSION: TGFβ and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis.

关 键 词：HL-60 cells HOMOHARRINGTONINE APOPTOSIS oligonucleotide array sequence analysis gene expression 

分 类 号：R966[医药卫生—药理学]