Estrogen influences the differentiation, maturation and function of dendritic cells in rats with experimental autoimmune encephalomyelitis  

作　　者：Qing-hongZHANG Yu-zhenHU JunCAO Yan-qingZHONG Yu-fengZHAO Qi-bingMEI 

机构地区：[1]CorrespondencetoDrQing-hongZHANG [2]DepartmentofPhysiology [3]DepartmentofOrthodontics [4]DepartmentofPharmacology,TheFourthMilitaryMedicalUniversity,Xi＇an710032,China

出　　处：《Acta Pharmacologica Sinica》2004年第4期508-513,共6页中国药理学报（英文版）

基　　金：Project partly supported by Technological Innovation Projectof the 4th Military Medical University (No CX02A024).

摘　　要：AIM: To examine if estrogen can affect the immune response at the dendriticcells (DCs) level in rats with experimental autoimmune encephalomyelitis (EAE). METHODS: Lewis ratswere immunized with inoculum containing MBP_(68-86). DCs were derived from spleen monocytes of EAErats with IL-4 and GM-CSF in presence of 17β-estradiol (E2). Nitric oxide (NO) was detected byGriess reagent. The surface markers and cytokines production of DCs were shown by flow cytometry.DCs were cocultured with MBP-specific T cells, [~3H]-TdR incoportation was used to reveal theantigen presentability, the supernatant of the coculture were collected to examine the cytokinessecretion by ELISA. RESULTS: E2 activated DCs by accelerating the maturation process characterizedby upregulation of MHC II and costimulating molecule B7-1, B7-2, drastic high expression of CD40.IFN-γ-produc-ing DCs were also elevated without any alteration of IL-10. Estradiol-treated DCs(E2-DCs) secreted more NO in the culture supernatant. By contrast, E2-DCs showed decreased antigenpresentation ability with reduced secretion of IFN-γ but no alteration of IL-10 in the coculturewith T cells. CONCLUSION: Estrogen can affect the differentiation, maturation and function of DCsfrom EAE rats, which may be attributed to its protection against EAE and the remission of multiplesclerosis patients in pregnancy.AIM: To examine if estrogen can affect the immune response at the dendriticcells (DCs) level in rats with experimental autoimmune encephalomyelitis (EAE). METHODS: Lewis ratswere immunized with inoculum containing MBP_(68-86). DCs were derived from spleen monocytes of EAErats with IL-4 and GM-CSF in presence of 17β-estradiol (E2). Nitric oxide (NO) was detected byGriess reagent. The surface markers and cytokines production of DCs were shown by flow cytometry.DCs were cocultured with MBP-specific T cells, [~3H]-TdR incoportation was used to reveal theantigen presentability, the supernatant of the coculture were collected to examine the cytokinessecretion by ELISA. RESULTS: E2 activated DCs by accelerating the maturation process characterizedby upregulation of MHC II and costimulating molecule B7-1, B7-2, drastic high expression of CD40.IFN-γ-produc-ing DCs were also elevated without any alteration of IL-10. Estradiol-treated DCs(E2-DCs) secreted more NO in the culture supernatant. By contrast, E2-DCs showed decreased antigenpresentation ability with reduced secretion of IFN-γ but no alteration of IL-10 in the coculturewith T cells. CONCLUSION: Estrogen can affect the differentiation, maturation and function of DCsfrom EAE rats, which may be attributed to its protection against EAE and the remission of multiplesclerosis patients in pregnancy.

关 键 词：multiple sclerosis experimental autoimmune encephalomyelitis DENDRITICCELLS ESTROGENS nitric oxide 

分 类 号：R977.12[医药卫生—药品] R744.3[医药卫生—药学]