大鼠视网膜缺血再灌注后IL-1β的免疫组化研究  

The study of interleukin-1β immunohistochemistry in the retina of rat after retinal ischemia reperfusion

作　　者：杨云波[1] 丁正平[2] 吕源淑[2] 陈多恩[3] 

机构地区：[1]武警湖北总队医院眼科,武汉430061 [2]华中科技大学同济医学院附属医院协和医院眼科,武汉430022 [3]华中科技大学同济医学院免疫组化教研室,武汉430032

出　　处：《武警医学院学报》2003年第4期253-255,I002,共4页Acta Academiae Medicinae CPAPF

摘　　要：目的 :观察大鼠视网膜缺血再灌注 (retinalischemiareperfusion ,RIR)后 ,白细胞介素 1β (IL 1β)多肽在视网膜表达变化。方法 :采用前房灌注生理盐水 ,形成 130mmHg (17 3kPa)高眼压 ,诱导大鼠视网膜缺血 6 0min ,解除高眼压 ,建立RIR模型。缺血 6 0min ,再灌注 12h、 48h作视网膜冰冻切片 ,IL 1β免疫组化观察。结果 :正常对照组未见IL 1β表达 ,RIR后 12h、 48h视网膜神经节细层可见IL 1β表达。 结论 :IL 1β多肽在蛋白质水平参与RIR损伤发生。Objective:To determine in rats whether there is a change in interleukin-1β polypetide expression in retina after retinal ischemia reperfusion.Methods:Retinal ischemia was induced in SD rats by raising the intraocular pressure to 130mmHg(17.3kPa) for 60 min.Via cannulation into the anterior chamber,the model of retina ischemia reperfusion was established once releasing the pressure in the rat.The expression of IL-1β in cryostat sections of retina was observed at 12h、48h after 60min ischemia.Results:At 12h、48h after retina ischemia reperfusion the expression of IL-1β in the ganglion cell layer increased obviously,No expression was detected in the control group.Conclusion:It suggests that IL-1β polypeptide involves with RIR Injury.

关 键 词：视网膜 缺血再灌注 白细胞介素1Β 

分 类 号：Q25[生物学—细胞生物学] R744.1[医药卫生—神经病学与精神病学]