低强度激光对兔实验性牙齿移动牙槽骨组织中骨钙素水平变化的影响  

Osteocalcin Expression of Alveolar Bone Tissues by Low Power Laser Irradiation During Experimental Tooth Movement in Rabbits

作　　者：孙新华[1] 刘淑华[1] 

机构地区：[1]吉林大学口腔医学院口腔正畸科,长春市130041

出　　处：《中国激光医学杂志》2004年第1期30-32,共3页Chinese Journal of Laser Medicine & Surgery

摘　　要：目的观察低强度激光照射对兔实验性牙齿移动牙槽骨组织中骨钙素水平变化的影响。方法30只雄性大耳白兔,随机分为1、3、5、7、14和21d组,共6组,每组5只。在兔左右上颌第一磨牙与上颌切牙之间放置不锈钢闭合螺旋弹簧,牵引第一、二磨牙向近中移动。右侧为实验侧,以2.5J/cm2的He-Ne激光和514J/cm2的CO2激光同时照射。左侧为对照侧,不照射激光。拔除上颌第一磨牙,分别取其近、远中牙槽骨组织(根上2/3处),以放射免疫法检测牙槽骨组织中的骨钙素含量。结果低强度激光照射侧张力区骨钙素含量在施力1、3、5d组高于对照侧(P<0.05);压力区骨钙素含量以施力7d组最高,3、5d组照射侧骨钙素含量低于对照侧(P<0.05)。结论骨钙素在实验性正畸牙齿移动过程中参与了破骨与成骨活动,低强度激光照射可影响正畸牙周组织中骨钙素水平的变化,对正畸牙周组织的骨改建有促进作用。Objective To study the change in osteocalcin expression level in alveolar bone tissue by radioimmunoassay during experimental tooth movement in rabbits after low power laser irradiation. Methods 30 male rabbits were separated into 6 groups randomly,1 d,3 d,5 d,7 d,14 d and 21 d with 5 rabbits in each group. The coil spring which moved mesially the first molar was ligated between the maxillary first molar and incisor. The right side was designed with irradiated side by low power laser and the left side was considered as a control side , in the self control. After pulling out the maxillary first molar, the mesial and distal alveolar bone tissues were collected and tested by radioimmunoassay methods and analysis by statistic t test. Results The osteocalcin expression of irradiated side by low power laser was higher than no irradiated side during 1 d,3 d,5 d in the tension zones, P<0.05. The osteocalcin expression of no irradiated side was higher than irradiated side by low power laser during 3 d,5 d in the pression zones, P<0.05. Conclusions Osteocalcin takes part in the bone remodeling during experimental orthodontic tooth movement. The osteocalcin′ s express is enhanced and the bone remodeling in periodontal tissue is promoted through low power laser irradiation during orthodontic tooth movement.

关 键 词：低强度激光 兔 实验 牙齿移动 牙槽骨 骨钙素 正畸疗法 

分 类 号：R783.5[医药卫生—口腔医学] R454.2[医药卫生—临床医学]