核因子κB在人卵巢癌血管生成中的作用及机制  

Role and Mechanism of Nuclear Factor κB in Angiogenesis of Human Ovarian Carcinoma

作　　者：杨丽华[1] 王凯峰 周轶萍 李玛琳 

机构地区：[1]昆明医学院第二附属医院妇产科,云南昆明650101 [2]云南省药物药理重点实验室,云南昆明650101

出　　处：《癌症》2004年第5期531-534,共4页Chinese Journal of Cancer

摘　　要：背景和目的:有研究表明核因子κB(nuclearfactorκB,NFκB)与体外血管生成有关,但其与卵巢癌血管生成的关系未见报道。本研究旨在探讨NFκB在鸡胚尿囊膜(chickchorioallantcicmembrane,CAM)上人卵巢癌(humanovariancarcinoma,HOC)血管生成中的作用及作用机制。方法:将指数生长期的HOC细胞株TYK细胞以1×109/只鸡胚接种于CAM以建立HOCCAM模型。1、20只鸡胚应用ELISA法检测接种细胞后0h、6h、12h、24h、48h、72h时间点NFκB、bFGF、αⅤβ3的水平;2、检测NFκB、bFGF和αⅤβ3在CAM上人卵巢癌血管生成过程中的水平及其对血管生成的影响。另将80只鸡胚分为6组,细胞接种后6h,不同组CAM上分别加入生理盐水(normalsaline,NS)(对照组,16只)、抗NFκB(A组,16只)、抗bFGF(B组,16只)、抗NFκB+抗bFGF(D组,8只)、接种后12h加入抗αⅤβ3(C组,16只)和抗NFκB+抗αⅤβ3(E组,8只)抗体。加抗体后48hNS、A、B、C组各取出8只鸡胚,用ELISA检测CAM中NFκB、bFGF和αⅤβ3的水平。其余的鸡胚孵育5天,用图像分析法检测各组CAM血管面积比(vesselarea/area,VA/A)。结果:人卵巢癌CAM模型上NFκB、bFGF水平自6h、αⅤβ3水平自接种后12h开始显著增加(P<0.01)(0.185±0.01、0.771±0.16、0.231±0.02)。BACKGROUND &OBJECTIVE: It had been reported that nuclear factor κb (NFκB) was associated with in vitro angiogenesis, but its role in angiogenesis of human ovarian carcinoma (HOC) had not been reported yet. The objective of this paper was to investigate the role and the mechanism of NFκB in HOC angiogenesis in chick chorioallantcic membrane (CAM). METHODS: The HOC TYK cells were implanted into 10 day CAM by 1×109/per egg to establish the HOC CAM model. (1) With 20 eggs, the levels of NFκB, integrin αⅤβ3, basic fibroblast growth factor (bFGF) at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h time point after cells implanted were determined with ELISA. (2) The relationship among NFκB, bFGF,αⅤβ3 and the effect of them on HOC angiogenesis in CAM were determined. Another 80 eggs were divided into 6 groups, normal saline (NS) (control group, 16 eggs), anti NFκB (A group, 16 eggs), anti bFGF (B group, 16 eggs), anti NFκB +anti bFGF(D group, 8 eggs) were added respectively into CAM at 6h time point after the cells implanted, and then anti integrin αⅤβ3 (C group, 16 eggs), anti NFκB +anti αⅤβ3 (E group, 8 eggs) antibodies were added at 12h time point. Eight eggs from NS, A, B, C groups were taken out to determine the levels of NFκB, αⅤβ3, bFGF at 48h after the antibodies were added. The other eggs were incubated up to 5 days, and the vessel area/area were determined with image analysis. RESULTS: (1)The levels of NFκB and bFGF increased significantly at 6h, and that of Ⅴβ3 increase significantly at 12 h time point after implantation (P< 0.01) (0.185±0.01, 0.771±0.16, 0.231±0.02), the peaks of the three levels were reached at 48h (P< 0.01) (0.337±0.01, 1.639±0.01, 0.349±0.01). The levels of bFGF was significantly higher than those of NFκB and αⅤβ3 at each time point (P< 0.01), but there were no significant difference between NFκB and αⅤβ3 (P >0.05). (2)The levels of NFκB,bFGF, and αⅤβ3 in A and B groups were significantly lower than those of NS group (P< 0.01) (0.098±0.02, 0.

关 键 词：核因子KB 人 卵巢癌 血管生成 作用机制 

分 类 号：R737.31[医药卫生—肿瘤]