HPC2基因与宫颈癌关系的研究  

Study on the relationship between cervical carcinoma and HPC2 gene

作　　者：秦鸿雁[1] 王林[2] 杨红[2] 韩骅[1] 

机构地区：[1]第四军医大学基础部医学遗传学和发育生物学教研室,陕西西安710033 [2]第四军医大学西京医院妇产科,陕西西安710033

出　　处：《第四军医大学学报》2004年第2期166-168,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (30 2 0 0 1 4 8)

摘　　要：目的 :研究宫颈癌的发生与HPC2基因的关系 .方法 :利用PCR方法 ,以宫颈癌组织和Hela细胞基因组DNA为模板扩增人HPC2基因片段 ,将此基因片段插入pGEM Teasy载体 ,限制性内切酶酶切鉴定 ,测定序列 .结果 :从宫颈癌组织和Hela细胞中成功扩增了HPC2基因 ,Blast分析发现其C端一段保守序列具有多态性 .结论AIM: To study the relationship between the cervical carcinoma and HPC2 (human polycomb protein2, HPC2) gene. METHODS: HPC2 gene fragments were amplified using PCR from human cervical carcinoma samples and Hela cells. PCR products were cloned into pGEM Teasy vector, identified by digestion, and correct clones were sequenced. RESULTS: HPC 2 gene fragments were successfully obtained from human cervical carcinoma samples and Hela cells. Nucleotide homology analysis indicated that a conserved sequence in the C terminus of HPC 2 may have a polymorphism in the cervical carcinoma and Hela cells. CONCLUSION: Polymorphism within this motif of HPC2 may be involved in cervical carcinogenesis.

关 键 词：HPC2 克隆 分子 序列分析 宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤] Q756[医药卫生—临床医学]