大鼠局灶性脑缺血再灌注后CAD表达与细胞凋亡的关系  

Relationship between expression of Caspase-activated DNase gene and neuronal apoptosis during focal reversible ischemia in rat brain

作　　者：张莹[1] 于永发[1] 樊丽超[1] 黄湘楠[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院神经科,黑龙江哈尔滨150086

出　　处：《中风与神经疾病杂志》2004年第1期13-15,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的　探讨大鼠局灶性脑缺血再灌注后缺血半暗带 Caspase-3激活的 DNA酶 (CAD)基因的表达变化与细胞凋亡的关系。方法　线栓法建立大鼠大脑中动脉闭塞 (MCAO)及再通模型。应用 RT-PCR技术检测MCAO再通后不同时相缺血半暗带皮质 CAD基因的表达 ,同时利用 TU NEL法观察对应区域细胞凋亡的动态变化规律。结果　脑缺血再灌注 6h,半暗带皮质 CAD m RNA显著升高 ,密度比值为 0 .74± 0 .0 4,再灌注 2 4h达到高峰 (1.13± 0 .11)。对应各时相均可见神经细胞凋亡 ,凋亡细胞以再灌注 48h组为最高 (113 .10± 13 .88)。结论　脑缺血再灌注可致 CAD基因表达上调 。Objective To study the relationship between expression of Caspase-activated DNase (CAD) gene and neuronal apoptosis in ischemic penumbra after rat focal ischemia reperfusion after unilateral middle cerebral artery occ lusion. MethodsThe unilateral middle cerebral arteries of Wistar rats were occluded for 2h by an intraluminal filament and recirculation was car ried out for 6h,12h,24h,48h and 72h postoperatively. Reverse transcription polymerase chain reaction (RT -PCR) was applied to evaluate the CAD mRNA expression in the ischemic penumbra tissue ,respectively. Meanwhile,the neuronal apoptosis was detected by using a terminal deoxynucleotidyl-transferase mediated dUTP-biotin nick end labeling (TUNEL) in the penumbra. ResultsCAD mRNA increased since 6h after isch emic-reperfusion and peaked at 24h of reperfusion in the penumbra. Cell apoptos is occured in all groups of ischemic-reperfusion,the percentage of apoptosis cells in 48h reperfusion grou p was the highest among all of the groups. ConclusionThese re sults indicate that CAD gene expression may be subjected to up-regulation after cerebral ischemia and may play an important role in the course of neuronal apoptosis.

关 键 词：大鼠 局灶性脑缺血 再灌注 CAD表达 细胞凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]