胰腺癌相关SC6抗原的纯化及其特性  

PURIFICATION AND CHARACTERISTIC OF SC6 ANTIGEN ASSOCIATED PANCREATIC CANCER

作　　者：郭晓钟[1] 安戎[1] 赵学良[1] 张宁[1] 刘民培[2] 马世英[2] 

机构地区：[1]沈阳军区总医院消化科 [2]沈阳军区总医院医学实验科

出　　处：《肿瘤》1992年第3期119-120,共2页Tumor

摘　　要：通过Protein A-Sepharose 4B柱纯化的单克隆抗体SC6与溴化氢活化琼脂糖4B偶联,经亲和层析从可溶性结肠癌组织中得到纯化的SC6抗原。SDS-PAGE和免疫印迹等方法证实SC6抗原是糖蛋白。免疫组化显示34例胰腺癌中31例染色阳性。通过免疫放射分析法检测胰腺癌血清SC6抗原敏感性达70.72％(29/41),结果提示SC6抗原对于胰腺癌的诊断有一定临床意义。The McAb SC6 was purified by passing through a protein A-Sepharose 4B columnand covalently coupled with CNBr-activated Sepharose 4B. The results of SDS-PAGE,immunoblotting studies and others confirmed that the colonic cancer SC6 antigen isa glycoprotein. 31/34 tissues of pancreatic cancer were positively stained by ABCmethod. The sensitivity of sera for patients with pancreatic cancer was 70.72%(29/41)using an immunoradioassay.

关 键 词：胰腺肿瘤 SC6 抗原 

分 类 号：R735.903[医药卫生—肿瘤]